Jiang Hao, Li Fuhua, Zhang Jiquan, Zhang Jinkang, Huang Bingxin, Yu Yang, Xiang Jianhai
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China.
Mar Biotechnol (NY). 2014 Feb;16(1):111-23. doi: 10.1007/s10126-013-9538-8. Epub 2013 Sep 22.
Fenneropenaeus chinensis (Chinese shrimp) culture industry, like other Penaeidae culture, has been seriously affected by the shrimp diseases caused by bacteria and virus. To better understand the mechanism of immune response of shrimp to different pathogens, proteome research approach was utilized in this study. Firstly, the soluble hepatopancreas protein samples in adult Chinese shrimp among control, heat-inactivated Vibrio-challenged and white spot syndrome virus-infected groups were separated by 2-DE (pH range, 4-7; sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and pH range, 3-10; tricine-SDS-PAGE). Then the differentially expressed protein spots (≥1.5-fold or ≤0.67-fold averagely of controls) were analyzed by LC-ESI-MS/MS. Using Mascot online database searching algorithm and SEQUEST searching program, 48 and 49 differentially expressed protein spots were successfully identified in response to Vibrio and white spot syndrome virus infection, respectively. Based on these results, we discussed the mechanism of immune response of the shrimp and shed light on the differences between immune response of shrimp toward Vibrio and white spot syndrome virus. This study also set a basis for further analyses of some key genes in immune response of Chinese shrimp.
中国对虾养殖产业，与其他对虾科养殖一样，受到细菌和病毒引发的虾类疾病的严重影响。为了更好地理解对虾对不同病原体的免疫反应机制，本研究采用了蛋白质组学研究方法。首先，通过双向电泳（pH范围4 - 7；十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS - PAGE））以及pH范围3 - 10；三羟甲基氨基甲烷 - SDS - PAGE），分离了对照组、热灭活弧菌刺激组和感染白斑综合征病毒的成年中国对虾的可溶性肝胰腺蛋白质样品。然后，对差异表达的蛋白质点（相对于对照组平均≥1.5倍或≤0.67倍）进行液相色谱 - 电喷雾串联质谱（LC - ESI - MS/MS）分析。使用Mascot在线数据库搜索算法和SEQUEST搜索程序，分别成功鉴定出48个和49个响应弧菌和白斑综合征病毒感染的差异表达蛋白质点。基于这些结果，我们讨论了对虾的免疫反应机制，并阐明了对虾对弧菌和白斑综合征病毒免疫反应的差异。本研究也为进一步分析中国对虾免疫反应中的一些关键基因奠定了基础。
