Honma Kazue, Mochizuki Kazuki, Goda Toshinao
Laboratory of Nutritional Physiology, Graduate School of Nutritional and Environmental Sciences and Global COE, The University of Shizuoka, Shizuoka, Japan.
Nutrition. 2009 Jan;25(1):40-4. doi: 10.1016/j.nut.2008.07.006. Epub 2008 Oct 26.
We examined in this study whether histone H3 acetylations on jejunal NA(+)/glucose transport-1 (SGLT1) gene are associated with induction of its gene by feeding mice a high carbohydrate/fat ratio diet.
The chromatin immunoprecipitation (ChIP) assays using antibodies of acetylated histone H3 were performed in jejunum of mice fed a high carbohydrate/fat ratio diet or a low carbohydrate/fat ratio for 7 d.
The acetylations of histone H3 of lysine 9/14 were highly detected on the SGLT1 gene and the levels were enhanced by feeding mice a high carbohydrate/fat ratio diet. Histone H3 acetylation at lysine 9 was enhanced by feeding a high carbohydrate/fat ratio diet, whereas that at lysine 14 was not enhanced significantly.
These observations indicate that induction of SGLT1 gene expression by feeding a high carbohydrate/fat ratio diet is associated with acetylation of histone H3 at lysine 9 on the SGLT1 gene.
在本研究中，我们探究了给小鼠喂食高碳水化合物/脂肪比例饮食时，空肠钠/葡萄糖转运体1（SGLT1）基因上的组蛋白H3乙酰化是否与其基因诱导有关。
使用乙酰化组蛋白H3抗体进行染色质免疫沉淀（ChIP）分析，实验对象为分别喂食高碳水化合物/脂肪比例饮食或低碳水化合物/脂肪比例饮食7天的小鼠空肠。
在SGLT1基因上高度检测到赖氨酸9/14的组蛋白H3乙酰化，并且通过给小鼠喂食高碳水化合物/脂肪比例饮食，其水平会升高。喂食高碳水化合物/脂肪比例饮食可增强赖氨酸9处的组蛋白H3乙酰化，而赖氨酸14处的乙酰化没有显著增强。
这些观察结果表明，通过喂食高碳水化合物/脂肪比例饮食诱导SGLT1基因表达与SGLT1基因上赖氨酸9处的组蛋白H3乙酰化有关。
