Mazeĭka A N, Popov A M, Kalinin V I, Avilov S A, Sil'chenko A S, Kostetskiĭ E Ia
Biofizika. 2008 Sep-Oct;53(5):826-35.
The ability of some triterpene glycosides of holothurians: cucumarioside A2-2 from Cucumaria japonica, cucumarioside G1 from Cucumaria fraudatrix, frondoside A from Cucumaria frondosa, and holotoxin A1 from A postichopus japonicus to form lipid-saponin supramolecular complexes was studied. The formation of supramolecular cholesterol-glycosides complexes between cholesterol and these glycosides in water medium was observed by transmission electron microscopy. These complexes were considered as nanoparticles with different structure. Complexes formed by cholesterol with cucumarioside A2-2, holotoxin A1, and frondoside A are tubular nanoparticles. In contrast, complexes between cholesterol and cucumarioside G1 have different structured. The structure of nanoparticles formed in the presence of cucumarioside A2-2, holotoxin A1, and cucumarioside G1 was dependent on the ratio of cholesterol in the lipid-saponin system. On the other hand, frondoside A did not shown this tendency. In lipid-saponin systems with a similar molar ratio cholesterol-glycoside, the ordering of the supramolecular structure decreases in the following order: cucumarioside A2-2, holotoxin A1, frondoside A. A comparative analysis of the morphology of the supramolecular complexes and the peculiarities of the molecular structure of triterpene glycosides studied, demonstrated that the structure of supramolecular complexes formed depends on the branching and length of the glycoside carbohydrate chain. On the other hand, the formation of monomeric cholesterol-glycosides complexes depends on the peculiarities of the structure of aglycone. Thus, the possibility of the formation of a new type of antigen carries on the basis of marine triterpene glycosides was proved.
来自日本瓜参的海参苷A2-2、来自伪刺参的海参苷G1、来自瘤瓜参的叶状皂苷A以及来自日本刺参的海参毒素A1形成脂质-皂苷超分子复合物的能力。通过透射电子显微镜观察了胆固醇与这些糖苷在水介质中形成超分子胆固醇-糖苷复合物的情况。这些复合物被认为是具有不同结构的纳米颗粒。胆固醇与海参苷A2-2、海参毒素A1和叶状皂苷A形成的复合物是管状纳米颗粒。相比之下，胆固醇与海参苷G1之间的复合物具有不同的结构。在海参苷A2-2、海参毒素A1和海参苷G1存在下形成的纳米颗粒的结构取决于脂质-皂苷体系中胆固醇的比例。另一方面，叶状皂苷A没有表现出这种趋势。在胆固醇-糖苷摩尔比相似的脂质-皂苷体系中，超分子结构的有序性按以下顺序降低：海参苷A2-2、海参毒素A1、叶状皂苷A。对所研究的三萜糖苷的超分子复合物形态和分子结构特点进行的比较分析表明，形成的超分子复合物的结构取决于糖苷碳水化合物链的分支和长度。另一方面，单体胆固醇-糖苷复合物的形成取决于苷元结构的特点。因此，证明了基于海洋三萜糖苷形成新型抗原的可能性。
