Ishihara Masashi, Kojima Ryoji, Ito Mikio
Laboratory of Analytical Pharmacology, Faculty of Pharmacy, Meijo University, 150 Yagotoyama, Tenpaku-ku, Nagoya 468-8503, Japan.
Eur J Pharmacol. 2008 Dec 28;601(1-3):143-7. doi: 10.1016/j.ejphar.2008.10.020. Epub 2008 Oct 21.
In the present study, we compared the effects of alpha-tocopherol and probucol, antioxidants, on the healing of acetic acid-induced gastric ulcers in 8-, 48- and 96-week-old rats. The repeated oral administration of alpha-tocopherol (16 mg/kg twice daily) and probucol (1000 mg/kg twice daily) for 14 consecutive days markedly accelerated the gastric ulcer healing in 48- and 96-week-old rats as well as 8-week-old ones. The ulcer healing effects of both drugs were not significantly different among the rats at three different ages. The superoxide dismutase (SOD) activity in the ulcerated region of 8-, 48- and 96-week-old rats was markedly lower than that in the unulcerated region. In contrast, the thiobarbituric acid (TBA)-reactive substance content, an index of lipid peroxidation, in the ulcerated region of rats at three different ages markedly increased, as compared to that in the unulcerated region. The SOD activity tended to decrease with aging, while the TBA-reactive substance content gradually increased. The repeated administration of alpha-tocopherol and probucol accelerated the ulcer healing and inhibited the increase in the TBA-reactive substance content in the ulcerated region. These results suggest that alpha-tocopherol and probucol promote the ulcer healing by their potent antioxidant activities in 48- and 96-week-old rats as well as 8-week-old rats.
在本研究中，我们比较了抗氧化剂α-生育酚和普罗布考对8周龄、48周龄和96周龄大鼠醋酸诱导胃溃疡愈合的影响。连续14天重复口服α-生育酚（16毫克/千克，每日两次）和普罗布考（1000毫克/千克，每日两次）可显著加速48周龄和96周龄大鼠以及8周龄大鼠的胃溃疡愈合。在三个不同年龄的大鼠中，两种药物的溃疡愈合效果没有显著差异。8周龄、48周龄和96周龄大鼠溃疡区域的超氧化物歧化酶（SOD）活性明显低于未溃疡区域。相反，与未溃疡区域相比，三个不同年龄大鼠溃疡区域的硫代巴比妥酸（TBA）反应性物质含量（脂质过氧化指标）显著增加。SOD活性倾向于随年龄增长而降低，而TBA反应性物质含量逐渐增加。重复给予α-生育酚和普罗布考可加速溃疡愈合，并抑制溃疡区域TBA反应性物质含量的增加。这些结果表明，α-生育酚和普罗布考通过其强大的抗氧化活性促进48周龄和96周龄大鼠以及8周龄大鼠的溃疡愈合。
