Ibrahim Faten M, Attia Hanan Naeim, Maklad Yousreya Aly Aly, Ahmed Kawkab A, Ramadan Mohamed F
a Department of Medicinal and Aromatic Plants Research, Pharmaceutical and Drug Industries Division , National Research Centre , Dokki , Giza , Egypt.
b Department of Medicinal and Pharmaceutical Chemistry (Pharmacology group) , Pharmaceutical and Drug Industries Research Division, National Research Centre , Dokki , Giza , Egypt.
Pharm Biol. 2017 Dec;55(1):740-748. doi: 10.1080/13880209.2016.1275705.
Cold-pressed oils (CPO) are commercially available in the market and characterized by their health-promoting properties.
Clove oil (CLO), coriander seed oil (COO) and black cumin oil (BCO) were evaluated for their bioactive lipids. Pharmacological screening was performed to evaluate acute toxicity, anti-inflammatory and ulcerogenic effects as well as histopathological changes in tissues of albino rats fed with CPO.
Fatty acids, tocols and total phenolics were analyzed. The acute toxicity test for each CPO was estimated during 14 d. Carrageenan-induced rat paw oedema was used for assessment of anti-inflammatory activity of CPO. Animals were fasted overnight, and via oral gavage given indomethacin (10 mg/kg) or CPO (400 mg/kg) to investigate ulcerogenecity. Histopathological changes in liver, kidney, heart, spleen and stomach were screened.
Amounts of α-, β-, γ- and δ-tocopherols in CLO were 1495, 58, 4177 and 177 mg/kg oil, respectively. In COO, α, β, γ and δ-tocopherols were 10.0, 18.2, 5.1 and 34.8%, respectively. In BCO, β-tocotrienol was the main constituent. CLO, COO and BCO contained 4.6, 4.2 and 3.6 mg GAE/g, respectively. Acute toxicity test determined that 400 mg/kg of CPO to be used. In the carrageenan model of inflammation, pretreatment of rats with indomethacin (10 mg/kg) or CLO (400 mg/kg) induced a significant (p < 0.05) reduction by 31.3 and 27.4%, respectively, in rat paw oedema as compared with the carrageenan-treated group. Indomethacin induced a significant ulcerogenic effect with an ulcer index of 19. Oral treatment of CPO showed no ulcerogenic effect, wherein no histopathological changes were observed.
CPO, particularly CLO, could minimize acute inflammation.
冷榨油（CPO）在市场上可商业购得，具有促进健康的特性。
评估丁香油（CLO）、芫荽籽油（COO）和黑孜然油（BCO）的生物活性脂质。进行药理筛选以评估急性毒性、抗炎和致溃疡作用以及喂食CPO的白化大鼠组织中的组织病理学变化。
分析脂肪酸、生育酚和总酚含量。在14天内评估每种CPO的急性毒性试验。角叉菜胶诱导的大鼠爪肿胀用于评估CPO的抗炎活性。动物禁食过夜，通过口服灌胃给予吲哚美辛（10mg/kg）或CPO（400mg/kg）以研究致溃疡作用。筛选肝脏、肾脏、心脏、脾脏和胃的组织病理学变化。
CLO中α-、β-、γ-和δ-生育酚的含量分别为1495、58、4177和177mg/kg油。在COO中，α、β、γ和δ生育酚分别为10.0%、18.2%、5.1%和34.8%。在BCO中，β-生育三烯酚是主要成分。CLO、COO和BCO分别含有4.6、4.2和3.6mg GAE/g。急性毒性试验确定使用400mg/kg的CPO。在角叉菜胶炎症模型中，与角叉菜胶处理组相比，用吲哚美辛（10mg/kg）或CLO（400mg/kg）预处理大鼠可使大鼠爪肿胀分别显著（p<0.05）降低31.3%和27.4%。吲哚美辛诱导显著的致溃疡作用，溃疡指数为19。口服CPO未显示致溃疡作用，其中未观察到组织病理学变化。
CPO，特别是CLO，可使急性炎症最小化。
