Kitasato Institute for Life Sciences & Graduate School of Infection Control Sciences, Kitasato University, Tokyo 108-8641, Oriental Medicine Research Center, The Kitasato Institute, Tokyo 108-8642 and Life Science Research Center, University of Toyama, Toyama 930-0194, Japan.
Evid Based Complement Alternat Med. 2010 Sep;7(3):331-40. doi: 10.1093/ecam/nen007. Epub 2008 Feb 27.
Kampo (traditional Japanese herbal) medicines are taken orally due to which the gastric mucosal immune system may act as one of the major targets for the expression of pharmacological activity. The inner surface of the intestinal tract possesses a large area of mucosal membranes, and the intestinal epithelial cells sit at the interface between a lumen and a lymphocyte-rich lamina propria. The cross talk that occurs between these compartments serves to maintain intestinal homeostasis, and the cytokine network plays an important role in the cross talk. In this study, the effect of Hochuekkito (HET), one of Kampo medicines, on cytokine secretion of intestinal epithelial cells was investigated. When murine normal colonic epithelial cell-line MCE301 cells were stimulated with HET, the contents of granulocyte colony-stimulating factor (G-CSF) in the conditioned medium were significantly increased in dose- and time-dependent manners. The enhanced G-CSF gene transcription in MCE301 cells by the stimulation of HET was observed by RT-PCR. The enhanced G-CSF secretion by HET was also observed in C3H/HeJ mice-derived primary cultured colonic epithelial cells. When the HET was fractionated, only the polysaccharide fraction (F-5) enhanced the G-CSF secretion of MCE301 cells, and the activity of F-5 lost after the treatment of periodate that can degrade the carbohydrate moiety. These results suggest that HET enhances secretion of G-CSF from colonic epithelial cells and the polysaccharide is one of the active ingredients of HET. The enhanced G-CSF secretion by HET may partly contribute to the clinically observed various pharmacological activities of HET including immunomodulating activity.
汉方药（传统的日本草药）通过口服给药，因此胃黏膜免疫系统可能成为表达药理活性的主要靶点之一。肠道的内表面具有大面积的黏膜，而肠上皮细胞位于腔和富含淋巴细胞的固有层之间的界面上。这些隔室之间发生的串扰有助于维持肠道内环境平衡，细胞因子网络在串扰中发挥重要作用。在这项研究中，研究了汉方药之一的黄耆建中汤（HET）对肠上皮细胞细胞因子分泌的影响。当用 HET 刺激鼠正常结肠上皮细胞系 MCE301 细胞时，条件培养基中粒细胞集落刺激因子（G-CSF）的含量呈剂量和时间依赖性显著增加。通过 RT-PCR 观察到 HET 刺激下 MCE301 细胞中 G-CSF 基因转录的增强。在 C3H/HeJ 小鼠来源的原代培养结肠上皮细胞中也观察到 HET 增强 G-CSF 的分泌。当将 HET 进行分段时，只有多糖部分（F-5）增强了 MCE301 细胞中 G-CSF 的分泌，并且 F-5 的活性在处理可以降解碳水化合物部分的过碘酸盐后丧失。这些结果表明，HET 增强了结肠上皮细胞中 G-CSF 的分泌，多糖是 HET 的活性成分之一。HET 增强的 G-CSF 分泌可能部分有助于 HET 的各种临床观察到的药理活性，包括免疫调节活性。
