Choi Jung Ho, Rho Mun-Chual, Lee Seung Woong, Choi Ji Na, Lee Hee Jeong, Bae Kyung Sook, Kim Koanhoi, Kim Young Kook
Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea.
J Microbiol Biotechnol. 2008 Oct;18(10):1663-5.
Acyl-coenzyme A: cholesterol acyltransferase (ACAT) catalyzes cholesterol esterification and plays an important role in the intestinal absorption of cholesterol, hepatic production of lipoproteins, and accumulation of cholesteryl ester within cells. During the course of screening to find ACAT inhibitors from microbial sources, the present authors isolated pyripyropene A from Penicillium griseofulvum F1959. Pyripyropene A, an ACAT2-specific inhibitor, has already been produced from Aspergillus fumigatus. Yet, Aspergillus fumigatus is a pathogen and only produces a limited amount of pyripyropene A, making the isolation of pyripyropene A troublesome. In contrast, Penicillium griseofulvum F1959 was found to produce approximately 28 times more pyripyropene A than Aspergillus fumigatus, plus this report also describes the ideal conditions for the production of pyripyropene A by Penicillium griseofulvum F1959 and its subsequent purification.
酰基辅酶A：胆固醇酰基转移酶（ACAT）催化胆固醇酯化，在胆固醇的肠道吸收、肝脏脂蛋白生成以及细胞内胆固醇酯的积累过程中发挥重要作用。在从微生物来源筛选ACAT抑制剂的过程中，作者从灰黄青霉F1959中分离出了吡咯并吡咯烯A。吡咯并吡咯烯A是一种ACAT2特异性抑制剂，已由烟曲霉产生。然而，烟曲霉是一种病原体，仅产生有限量的吡咯并吡咯烯A，这使得吡咯并吡咯烯A的分离很麻烦。相比之下，发现灰黄青霉F1959产生的吡咯并吡咯烯A比烟曲霉多约28倍，此外，本报告还描述了灰黄青霉F1959产生吡咯并吡咯烯A及其后续纯化的理想条件。
