Sheetz Kraig E, Hoover Erich E, Carriles Ramón, Kleinfeld David, Squier Jeff A
Department of Physics, Colorado School of Mines, Golden, Colorado 80401, USA.
Opt Express. 2008 Oct 27;16(22):17574-84. doi: 10.1364/oe.16.017574.
We present a novel Yb:KGd(WO(4))(2) oscillator design that generates six beams of temporally delayed, 253 fs, 11 nJ pulses. This allows multifocal nonlinear microscopy to be performed without the need for complicated optical multiplexers. We demonstrate our design with twelve simultaneously acquired two-photon, second-harmonic and/or third-harmonic images generated from six laterally separated foci.