Allende Motz Alyssa M, Czerski John, Adams Daniel E, Durfee Charles, Bartels Randy, Field Jeff, Hoy Christopher L, Squier Jeff
Opt Express. 2020 Jan 6;28(1):405-424. doi: 10.1364/OE.378460.
Spatial frequency modulated imaging (SPIFI) enables the use of an extended excitation source for linear and nonlinear imaging with single element detection. To date, SPIFI has only been used with fixed excitation source geometries. Here, we explore the potential for the SPIFI method when a spatial light modulator (SLM) is used to program the excitation source, opening the door to a more versatile, random access imaging environment. In addition, an in-line, quantitative pulse compensation and measurement scheme is demonstrated using a new technique, spectral phase and amplitude retrieval and compensation (SPARC). This enables full characterization of the light exposure conditions at the focal plane of the random access imaging system, an important metric for optimizing, and reporting imaging conditions within specimens.
空间频率调制成像（SPIFI）能够使用扩展激发源进行单元素检测的线性和非线性成像。迄今为止，SPIFI仅用于固定激发源几何形状。在此，我们探索当使用空间光调制器（SLM）对激发源进行编程时SPIFI方法的潜力，从而开启通向更通用的随机访问成像环境的大门。此外，使用一种新技术——光谱相位和幅度检索与补偿（SPARC）展示了一种在线定量脉冲补偿和测量方案。这能够全面表征随机访问成像系统焦平面处的光暴露条件，这是优化和报告样本内成像条件的一项重要指标。
