Rojas Enith I, Herre Edward Allen, Mejía Luis C, Arnold A Elizabeth, Chaverri Priscila, Samuels Gary J
Smithsonian Tropical Research Institute, Apartado Postal 0843-03092, Balboa, Ancon, Republic of Panama.
Mycologia. 2008 Sep-Oct;100(5):760-75. doi: 10.3852/07-207.
A new lineage is discovered within the Botryosphaeriaceae (Ascomycetes, Dothideomycetes, incertae sedis). Consistent with current practice of providing generic names for independent lineages, this lineage is described as Endomelanconiopsis gen. nov., with the anamorphic species E. endophytica sp. nov. and E. microspora comb. nov. (= Endomelanconium microsporum). Endomelanconiopsis is characterized by eustromatic conidiomata and holoblastically produced, brown, nonapiculate, unicellular conidia, each with a longitudinal germ slit. Phylogenetic analysis of partial sequences of LSU, ITS and translation elongation factor 1 alpha (tef1) indicate that E. endophytica is sister of E. microspora and that they are nested within the Botryosphaeriaceae. However because there is no support for the "backbone" of the Botryosphaeriacae we are not able to see the interrelationships among the many genera in the family. Neither species is known to have a teleomorph. Endomelanconiopsis differs from Endomelanconium because conidia of the type species of Endomelanconium, E. pini, are papillate at the base, conidiogenous cells proliferate sympodially and the pycnidial wall is thinner; we postulate that the teleomorph of E. pini as yet unknown is an inoperculate discomycete. Endomelanconiopsis endophytica was isolated as an endophyte from healthy leaves of Theobroma cacao (cacao, Malvaceae) and Heisteria concinna (Erythroplaceae) in Panama. Endomelanconiopsis microspora was isolated from soil in Europe.
在葡萄座腔菌科（子囊菌门，座囊菌纲，地位未定）内发现了一个新谱系。与当前为独立谱系提供属名的做法一致，该谱系被描述为新属内生黑盘孢菌属（Endomelanconiopsis gen. nov.），其无性型物种为新种内生内生黑盘孢菌（E. endophytica sp. nov.）和新组合小孢子内生黑盘孢菌（E. microspora comb. nov.）（= 小孢子内生黑盘菌（Endomelanconium microsporum））。内生黑盘孢菌属的特征为具真基质分生孢子器，分生孢子全壁芽生，褐色，无顶生突起，单细胞，每个都有一条纵向芽缝。对大亚基核糖体RNA（LSU）、内转录间隔区（ITS）和翻译延伸因子1α（tef1）部分序列的系统发育分析表明，内生内生黑盘孢菌是小孢子内生黑盘孢菌的姐妹种，且它们嵌套在葡萄座腔菌科内。然而，由于对葡萄座腔菌科的“主干”没有支持，我们无法看清该科众多属之间的相互关系。已知这两个物种均无有性型。内生黑盘孢菌属与内生黑盘菌属不同，因为内生黑盘菌属的模式种松果内生黑盘菌（E. pini）的分生孢子基部有乳头状突起，产孢细胞以合轴方式增殖，且分生孢子器壁较薄；我们推测松果内生黑盘菌未知的有性型是一种无孔盘菌。内生内生黑盘孢菌是从巴拿马的可可树（可可，锦葵科）和美丽海丝特木（红树科）的健康叶片中作为内生菌分离得到的。小孢子内生黑盘孢菌是从欧洲的土壤中分离得到的。
