Palestino Gabriela, Agarwal Vivechana, Aulombard Roger, Pérez Elías, Gergely Csilla
Groupe d'Etude des Semiconducteurs, UMR 5650, CNRS-Université Montpellier II, PI. Eugène Bataillon, 34095, Montpellier Cedex 5, France.
Langmuir. 2008 Dec 2;24(23):13765-71. doi: 10.1021/la8015707.
Porous silicon (PSi) is a promising biomaterial presenting the advantage of being biocompatible and bioresorbable. Due to the large specific surface area and unique optical features, these microporous structures are excellent candidates for biosensing applications. Investigating device functionality and developing simple Si-based transducers need to be addressed in novel biological detection. Our work demonstrates that, among the various PSi configurations for molecular detection, PSi microcavity structure demonstrates the best biosensing performance, reflected through the enhanced luminescence response and the changes in the refractive index. For successful immobilization, molecular infiltration and confinement are the two key factors that are controlled by the pore size distribution of the PSi microcavities and by the surface modification obtained by silane-glutaraldehyde chemistry. Enhancement of the fluorescence emission of confined fluorescent biomolecules in the active layer of PSi microcavities was observed for a nonlabeled protein with a natural green fluorescence, the glucose oxidase enzyme (GOX). An increase in the fluorescence emission was also observed when functionalized PSi material was used to detect specific binding between biotin and a low concentration of labeled streptavidin. Evidence for the enzymatic activity of GOX in its adsorbed form is also presented. Use of smart silicon devices, enabling enhancement of fluorescence emission of biomolecules, offers easy-to-use biosensing, based on the luminescence response of the molecules to be detected.
多孔硅（PSi）是一种很有前景的生物材料，具有生物相容性和生物可吸收性的优点。由于其大的比表面积和独特的光学特性，这些微孔结构是生物传感应用的极佳候选材料。在新型生物检测中，需要解决研究器件功能和开发简单的硅基传感器的问题。我们的工作表明，在用于分子检测的各种PSi结构中，PSi微腔结构表现出最佳的生物传感性能，这通过增强的发光响应和折射率的变化得以体现。对于成功的固定化，分子渗透和限制是两个关键因素，它们分别由PSi微腔的孔径分布以及通过硅烷 - 戊二醛化学获得的表面改性来控制。对于具有具有天然具有天然绿色荧光的非标记蛋白质葡萄糖氧化酶（GOX），观察到PSi微腔活性层中受限荧光生物分子的荧光发射增强。当使用功能化的PSi材料检测生物素与低浓度标记链霉亲和素之间的特异性结合时，也观察到荧光发射增加。还展示了吸附形式的GOX的酶活性证据。使用能够增强生物分子荧光发射的智能硅器件，基于待检测分子的发光响应，提供了易于使用的生物传感。
