Dipartimento di Scienze del Suolo, della Pianta, dell'Ambiente e delle Produzioni Animali, Università di Napoli Federico II, Via Università 100, 80055 Portici, Italy.
J Nat Prod. 2008 Nov;71(11):1897-901. doi: 10.1021/np800415w. Epub 2008 Oct 29.
Stagonospora cirsii Davis, a fungal pathogen isolated from Cirsium arvense (commonly called Canada thistle) and proposed as a potential mycoherbicide of this perennial noxious weed, produces phytotoxic metabolites in liquid and solid cultures. Stagonolide, the main phytotoxic metabolite, and five new related nonenolides, named stagonolides B-F, were isolated from the fungus. When grown on solid culture, nonenolide yields increased. A further four nonenolides were isolated and characterized by spectroscopy. Three were new compounds and named stagonolides G-I, and the fourth was identified as modiolide A, previously isolated from Paraphaeosphaeria sp., a fungus separated from the horse mussel. Leaf disk-puncture assays at 1 mg/mL of stagonolides H-I and modiolide A were phytotoxic to C. arvense. Only stagonolide H inhibited chicory seedling root growth. The most potent toxin, stagonolide H, indicated selectivity when tested on leaves of eight different plants: Canada thistle was most sensitive to the compound.
戴维斯叶点霉，一种从豚草（俗称加拿大蓟）中分离出来的真菌病原体，被提议作为这种多年生有害杂草的潜在真菌除草剂，在液体和固体培养物中产生植物毒性代谢物。叶点霉烯醇，主要的植物毒性代谢物，和五个新的相关非烯醇，命名为叶点霉烯醇 B-F，从真菌中分离出来。当在固体培养物上生长时，非烯醇的产量增加。通过光谱学进一步分离和表征了另外四种非烯醇。其中三种是新化合物，命名为叶点霉烯醇 G-I，第四种是命名为莫地奥内酯 A，先前从马贻贝分离出的 Phaeosphaeria sp. 真菌中分离出来的化合物。在 1mg/mL 的叶点霉烯醇 H-I 和莫地奥内酯 A 的叶盘刺孔试验中，对豚草叶片具有植物毒性。只有叶点霉烯醇 H 抑制菊苣幼苗根的生长。最有效的毒素叶点霉烯醇 H 在对 8 种不同植物的叶片进行测试时表现出选择性：豚草对该化合物最敏感。
