Barbas C F, Kang A S, Lerner R A, Benkovic S J
Department of Molecular Biology, Scripps Research Institute, La Jolla, CA 92037.
Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):7978-82. doi: 10.1073/pnas.88.18.7978.
A phagemid system was developed for the monovalent display of combinatorial antibody Fab libraries on the surface of filamentous phage M13. Fab fragments were fused to the carboxyl-terminal domain of the gene III protein. Phage displaying Fab fragments on their surface, or Phabs, were enriched by 10(3)- to 10(5)-fold on antigen-coated surfaces over nonspecific phage. The method may replace current antibody cloning techniques.
开发了一种噬菌粒系统，用于在丝状噬菌体M13表面单价展示组合抗体Fab文库。Fab片段与基因III蛋白的羧基末端结构域融合。在其表面展示Fab片段的噬菌体，即噬菌抗体，在抗原包被的表面上比非特异性噬菌体富集了10³至10⁵倍。该方法可能会取代目前的抗体克隆技术。
Zotero 插件
