环糊精增强荧光光谱法测定药物和生物体液中的氟西汀。
Cyclodextrin enhanced spectrofluorimetric determination of fluoxetine in pharmaceuticals and biological fluids.
机构信息
Department of Analytical Chemistry and Food Technology, University of Castilla-La Mancha, E-13071 Ciudad Real, Spain.
出版信息
Talanta. 2002 Aug 23;58(2):301-9. doi: 10.1016/s0039-9140(02)00241-2.
The characteristics of host-guest complexation between methyl-beta-cyclodextrin and fluoxetine were investigated by fluorescence spectrometry. A 1:1 stoichiometry of the complex was established and association constant of 4.35x10(-3) M(-1) at 20 degrees C was calculated. A spectrofluorimetric method for the determination of fluoxetine, with a range of application between 40 and 1000 mug l(-1) was developed. Overall least squares regression was used to find the straight line that fitted the experimental data. The detection limit, according to the error propagation theory, was 9.6 mug l(-1) and the detection limit proposed by Clayton was 15.8 mug l(-1). Repeatability and relative standard deviation were also determined according to this theory, with satisfactory results. The method was successfully applied to the determination of fluoxetine in pharmaceuticals and biological fluids.
采用荧光光谱法研究了甲基-β-环糊精与氟西汀之间的主客体络合特性。建立了 1:1 的络合物化学计量比，并在 20°C 下计算出结合常数为 4.35x10(-3) M(-1)。开发了一种用于测定氟西汀的荧光光谱法，其应用范围在 40 至 1000 μg l(-1)之间。采用总体最小二乘法找到拟合实验数据的直线。根据误差传播理论，检测限为 9.6 μg l(-1)，而 Clayton 提出的检测限为 15.8 μg l(-1)。根据该理论还确定了重复性和相对标准偏差，结果令人满意。该方法成功应用于药物和生物流体中氟西汀的测定。
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