State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Tianshui Road 222, Lanzhou 730000, PR China.
Bioorg Med Chem Lett. 2011 Feb 15;21(4):1113-7. doi: 10.1016/j.bmcl.2010.12.125. Epub 2011 Jan 1.
The host-guest inclusion system of ethyl substituted β-cyclodextrin (DE-β-CD) with mangiferin (MA) was investigated by fluorescence spectra in solution. The results showed that the MA was encapsulated in the DE-β-CD's cavity to form a 2:1 stoichiometry host-guest inclusion complex (DE-β-CD/MA) and the inclusion constant (K=3.04×10(6)L(2)/mol(2)) was confirmed by the typical double reciprocal plots. Furthermore, several experimental conditions were optimized in order to obtain the maximum fluorescence signal. In addition, the thermodynamic parameters, Gibbs free energy (ΔG°), enthalpy change (ΔH°) and entropy change (ΔS°) of DE-β-CD/MA were obtained by the Van't Hoff equation. A spectrofluorimetric method for the determination of MA in solution in the presence of DE-β-CD was developed based on the remarkable enhancement of the fluorescence intensity of MA. The linear range was 2.00×10(-8)-7.00×10(-6)mol/L and the detection limit was 4.05×10(-9)mol/L. The proposed method was successfully applied to the analysis of MA in serum with the satisfactory result.
采用荧光光谱法研究了取代基为乙基的β-环糊精（DE-β-CD）与芒果苷（MA）的主客体包合体系。结果表明，MA 被包合在 DE-β-CD 的空腔中形成 2:1 的主客体包合物（DE-β-CD/MA），通过典型的双倒数图证实了包合常数（K=3.04×10(6)L(2)/mol(2))。此外，还优化了几个实验条件，以获得最大的荧光信号。此外，还通过范特霍夫方程获得了 DE-β-CD/MA 的热力学参数，吉布斯自由能（ΔG°）、焓变（ΔH°）和熵变（ΔS°）。在 DE-β-CD 的存在下，基于 MA 的荧光强度显著增强，建立了一种测定溶液中 MA 的荧光分光光度法。线性范围为 2.00×10(-8)-7.00×10(-6)mol/L，检测限为 4.05×10(-9)mol/L。该方法成功应用于血清中 MA 的分析，结果令人满意。
