Department of Chemistry, Punjabi University Patiala, 147002, Punjab, India.
J Fluoresc. 2012 Sep;22(5):1407-13. doi: 10.1007/s10895-012-1079-4. Epub 2012 Jun 24.
A simple, rapid and sensitive constant wavelength synchronous fluorescence method is developed for the determination of danofloxacin (DAN) in pharmaceutical formulations and its residue in milk based on Al(III) enhanced fluorescence. The synchronous fluorescence intensity of the system is measured at 435 nm using ∆ λ = 80 nm and an excitation wavelength of 280 nm. A good linear relationship between enhanced fluorescence intensity and DAN concentration is obtained in the range of 3-100 ng mL(-1)(r (2) = 0.9991). The limit of detection (LOD, S/N = 3) of the present method is 0.9 ng mL(-1). The proposed method can be successfully applied to the determination of DAN in pharmaceutical formulations and in milk without serious interferences from common excipients, metal ions and other co-existing substances. The method can be used as a rapid screening to judge whether the DAN residues in milk exceed Maximum Residue Limits (MRLs) or not.
建立了一种基于 Al(III)增强荧光的简单、快速、灵敏的同步波长荧光法，用于测定药物制剂中的丹诺沙星(DAN)及其在牛奶中的残留量。在 ∆ λ = 80nm 和激发波长为 280nm 的条件下，测定体系的同步荧光强度在 435nm 处增强。在 3-100ngmL(-1)(r(2)=0.9991)范围内，增强荧光强度与 DAN 浓度呈良好的线性关系。本方法的检出限(LOD，S/N = 3)为 0.9ngmL(-1)。该方法可成功应用于药物制剂和牛奶中 DAN 的测定，不受常见赋形剂、金属离子和其他共存物质的严重干扰。该方法可作为一种快速筛选方法，用于判断牛奶中 DAN 残留是否超过最大残留限量(MRLs)。
