基于奎宁 - 铈（IV）体系直接化学发光法测定人血清中的半胱氨酸
Direct chemiluminescence determination of cysteine in human serum using quinine-Ce(IV) system.
作者信息
Nie Lihua, Ma Huimin, Sun Ming, Li Xiaohua, Su Meihong, Liang Shuchuan
机构信息
Laboratory of Chemical Biology, Center for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China.
出版信息
Talanta. 2003 Apr 10;59(5):959-64. doi: 10.1016/S0039-9140(02)00649-5.
A simple, sensitive and selective chemiluminescence (CL) method was developed for the determination of cysteine. This method is based on that the weak CL of cysteine oxidized with cerium (IV) can be greatly enhanced by quinine. The calibration curve was linear over the range 3.5 x 10(-9)-3.5 x 10(-6) M with a detection limit of 2.5 x 10(-9) M (S/N=3). The RSD was found to be 8.4% by 10 replicate determinations of 2.9 x 10(-8) M cysteine. Due to high sensitivity, the proposed method can be used directly to determine the total concentration of cysteine in human serum through simply diluting the sample for a thousand fold. The obtained result was in agreement with that given by amino acid autoanalyzer. The present method does not require any separation, showing a simpler analytical characteristic. The mechanism of the CL reaction was also discussed.
开发了一种简单、灵敏且选择性好的化学发光（CL）法用于测定半胱氨酸。该方法基于用铈（IV）氧化半胱氨酸产生的微弱化学发光能被奎宁大大增强。校准曲线在3.5×10⁻⁹ - 3.5×10⁻⁶ M范围内呈线性，检测限为2.5×10⁻⁹ M（S/N = 3）。对2.9×10⁻⁸ M半胱氨酸进行10次重复测定，相对标准偏差为8.4%。由于灵敏度高，该方法可通过将样品简单稀释一千倍直接用于测定人血清中半胱氨酸的总浓度。所得结果与氨基酸自动分析仪给出的结果一致。本方法无需任何分离，显示出更简单的分析特性。还讨论了化学发光反应的机理。
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