Chemistry Department, Faculty of Science, Al-Azhar University, Assiut Branch, 71524 Assiut, Egypt.
Talanta. 2004 Feb 27;62(3):531-8. doi: 10.1016/j.talanta.2003.08.026.
Azithromycin (AZ) is the first member of a class of macrolide azalides antibiotics called azolides. A simple and selective square-wave voltammetric (SWV) method has been developed for the determination of azithromycin in pure form, in pharmaceutical preparation and in biological samples. Determination of azithromycin was accomplished with hand-make carbon paste electrode (CPE) in oxidative screen mode. The counter and reference electrodes were a Pt wire and a Ag/AgCl, respectively. Various parameters that can influence the peak signal (effect of buffer, ionic strength, accumulation time, pH and the composition of the paste) have been scrutinized. The best results were obtained in acetonitrile-aqueous 1M sodium acetate-acetic acid buffer (pH 4.6) containing 0.1M KCl (1:9; v/v) using a 15% paraffin oil CPE. The limits of detection and quantification of the pure drug are 0.463 and 1.544ppb (with the correlation coefficient, r=0.9785and the standard deviation, S.D.=0.1 (n=5), for the accumulation time of 60s), respectively. The method was successfully applied to the determination of the drug in urine and two forms of pharmaceutical formulations. Recoveries were 99.2-100.5% with S.D.=0.1-and 0.8% (n=5).
阿奇霉素（AZ）是一类称为氮杂内酯的大环内酯类抗生素的第一个成员。已经开发出一种简单而选择性的方波伏安法（SWV），用于测定纯形式、药物制剂和生物样品中的阿奇霉素。在氧化筛选模式下，使用手工碳糊电极（CPE）来测定阿奇霉素。对电极和参比电极为 Pt 丝和 Ag/AgCl。已经研究了可以影响峰信号的各种参数（缓冲液、离子强度、积累时间、pH 值和糊剂的组成的影响）。在含有 0.1M KCl（1:9；v/v）的 1M 醋酸钠-乙酸缓冲液（pH 4.6）中，使用 15%的石蜡油 CPE 可以获得最佳结果。纯药物的检测限和定量限分别为 0.463 和 1.544ppb（在 60s 的积累时间下，相关系数 r=0.9785，标准偏差 S.D.=0.1（n=5））。该方法成功地应用于尿液和两种药物制剂中药物的测定。回收率为 99.2-100.5%，标准偏差为 0.1-0.8%（n=5）。
