Di Marzo V, Côté M, Matias I, Lemieux I, Arsenault B J, Cartier A, Piscitelli F, Petrosino S, Alméras N, Després J-P
Endocannabinoid Research Group, Istituto di Chimica Biomolecolare, Consiglio Nazionale delle Ricerche, Via Campi Flegrei 34, Comprensorio Olivetti, 80078 Pozzuoli (NA), Italy.
Diabetologia. 2009 Feb;52(2):213-7. doi: 10.1007/s00125-008-1178-6.
AIMS/HYPOTHESIS: We previously reported that the plasma levels of the endocannabinoid, 2-arachidonoylglycerol (2-AG), in a cohort of viscerally obese men are directly correlated with visceral adipose tissue (VAT) accumulation and metabolic risk factors including low HDL-cholesterol and high triacylglycerol. It is not known, however, if such correlations persist after vigorous lifestyle interventions that reduce metabolic risk factors. We analysed the changes in endocannabinoid levels in a subsample from the same cohort following a 1 year lifestyle modification programme, and correlated them with changes in VAT and metabolic risk factors.
Forty-nine viscerally obese men (average age 49 years, BMI 30.9 kg/m(2), waist 107.3 cm) underwent a 1 year lifestyle modification programme including healthy eating and physical activity. Plasma levels of 2-AG and the other most studied endocannabinoid, anandamide, were measured by liquid chromatography-mass spectrometry. Anthropometric and metabolic risk factors, including VAT, insulin resistance and glucose intolerance, HDL-cholesterol and triacylglycerol, were measured.
Most risk factors were improved by the intervention, which led to a significant decrease in body weight (-6.4 kg, p < 0.0001), waist circumference (-8.0 cm, p < 0.0001) and VAT (-30%, p < 0.0001), and in plasma 2-AG (-62.3%, p < 0.0001) and anandamide (-7.1%, p = 0.005) levels. The decrease in levels of 2-AG but not those of anandamide correlated with decreases in VAT and triacylglycerol levels, and with the increase in HDL(3)-cholesterol levels. Multivariate analyses suggested that decreases in 2-AG and VAT were both independently associated with decreases in triacylglycerol.
CONCLUSIONS/INTERPRETATION: This study shows that a strong correlation exists between 2-AG levels and high plasma triacylglycerol and low HDL(3)-cholesterol in viscerally obese men.
目的/假设：我们之前报道过，在一群内脏型肥胖男性中，内源性大麻素2-花生四烯酸甘油酯（2-AG）的血浆水平与内脏脂肪组织（VAT）堆积以及包括低高密度脂蛋白胆固醇和高三酰甘油在内的代谢风险因素直接相关。然而，在进行了能降低代谢风险因素的积极生活方式干预后，这种相关性是否仍然存在尚不清楚。我们分析了同一队列中的一个子样本在进行了为期1年的生活方式改善计划后内源性大麻素水平的变化，并将其与VAT和代谢风险因素的变化进行关联分析。
49名内脏型肥胖男性（平均年龄49岁，体重指数30.9kg/m²，腰围107.3cm）接受了为期1年的生活方式改善计划，包括健康饮食和体育活动。通过液相色谱-质谱法测量2-AG以及另一种研究最多的内源性大麻素花生四烯乙醇胺的血浆水平。测量人体测量学指标和代谢风险因素，包括VAT、胰岛素抵抗和葡萄糖耐量、高密度脂蛋白胆固醇和三酰甘油。
干预使大多数风险因素得到改善，导致体重显著下降（-6.4kg，p<0.0001）、腰围下降（-8.0cm，p<0.0001）和VAT下降（-30%，p<0.0001），同时血浆2-AG水平下降（-62.3%，p<0.0001），花生四烯乙醇胺水平下降（-7.1%，p=0.005）。2-AG水平的下降而非花生四烯乙醇胺水平的下降与VAT和三酰甘油水平的下降以及高密度脂蛋白3（HDL₃）胆固醇水平的升高相关。多变量分析表明，2-AG和VAT的下降均与三酰甘油的下降独立相关。
结论/解读：本研究表明，在内脏型肥胖男性中，2-AG水平与高血浆三酰甘油和低HDL₃胆固醇之间存在很强的相关性。
