葡糖苷酶II对N-聚糖的修剪对于拟南芥的发育至关重要。
N-glycan trimming by glucosidase II is essential for Arabidopsis development.
作者信息
Soussilane Pravina, D'Alessio Cecilia, Paccalet Thomas, Fitchette Anne-Catherine, Parodi Armando J, Williamson Richard, Plasson Carole, Faye Loïc, Gomord Véronique
机构信息
CNRS, UMR 6037, IFRMP 23, Bâtiment Biologie Extension, Faculté des Sciences, Mont-Saint-Aignan, France.
出版信息
Glycoconj J. 2009 Jul;26(5):597-607. doi: 10.1007/s10719-008-9201-1. Epub 2008 Oct 30.
Abstract
Glucosidase II, one of the early N-glycan processing enzymes and a major player in the glycoprotein folding quality control, has been described as a soluble heterodimer composed of alpha and beta subunits. Here we present the first characterization of a plant glucosidase II alpha subunit at the molecular level. Expression of the Arabidopsis alpha subunit restored N-glycan maturation capacity in Schizosaccharomyces pombe alpha- or alphabeta-deficient mutants, but with a lower efficiency in the last case. Inactivation of the alpha subunit in a temperature sensitive Arabidopsis mutant blocked N-glycan processing after a first trimming by glucosidase I and strongly affected seedling development.
摘要
葡糖苷酶II是早期N-聚糖加工酶之一，也是糖蛋白折叠质量控制中的主要参与者，它被描述为一种由α和β亚基组成的可溶性异二聚体。在此，我们首次在分子水平上对植物葡糖苷酶II的α亚基进行了表征。拟南芥α亚基的表达恢复了粟酒裂殖酵母α缺陷或αβ缺陷突变体中N-聚糖的成熟能力，但在后一种情况下效率较低。在温度敏感的拟南芥突变体中使α亚基失活，会在葡糖苷酶I首次修剪后阻断N-聚糖加工，并严重影响幼苗发育。
相似文献
1
N-glycan trimming by glucosidase II is essential for Arabidopsis development.葡糖苷酶II对N-聚糖的修剪对于拟南芥的发育至关重要。
Glycoconj J. 2009 Jul;26(5):597-607. doi: 10.1007/s10719-008-9201-1. Epub 2008 Oct 30.
2
Arabidopsis glucosidase I mutants reveal a critical role of N-glycan trimming in seed development.拟南芥葡糖苷酶I突变体揭示了N-聚糖修剪在种子发育中的关键作用。
EMBO J. 2001 Mar 1;20(5):1010-9. doi: 10.1093/emboj/20.5.1010.
3
Glucosidase II beta subunit modulates N-glycan trimming in fission yeasts and mammals.葡糖苷酶IIβ亚基调节裂殖酵母和哺乳动物中的N-聚糖修剪。
Mol Biol Cell. 2009 Sep;20(17):3974-84. doi: 10.1091/mbc.e09-04-0316. Epub 2009 Jul 15.
4
The cellulose-deficient Arabidopsis mutant rsw3 is defective in a gene encoding a putative glucosidase II, an enzyme processing N-glycans during ER quality control.缺乏纤维素的拟南芥突变体rsw3在一个编码假定葡糖苷酶II的基因中存在缺陷，该酶在内质网质量控制过程中加工N-聚糖。
Plant J. 2002 Dec;32(6):949-60. doi: 10.1046/j.1365-313x.2002.01483.x.
5
Requirement of a homolog of glucosidase II beta-subunit for EFR-mediated defense signaling in Arabidopsis thaliana.拟南芥中葡萄糖苷酶 IIβ亚基同源物对 EFR 介导的防御信号的要求。
Mol Plant. 2010 Jul;3(4):740-50. doi: 10.1093/mp/ssq017. Epub 2010 May 9.
6
Trimming of N-Glycans by the Golgi-Localized α-1,2-Mannosidases, MNS1 and MNS2, Is Crucial for Maintaining RSW2 Protein Abundance during Salt Stress in Arabidopsis.α-1,2-甘露糖苷酶 MNS1 和 MNS2 对 N-糖基化的修剪作用对于拟南芥盐胁迫过程中 RSW2 蛋白丰度的维持至关重要。
Mol Plant. 2018 May 7;11(5):678-690. doi: 10.1016/j.molp.2018.01.006. Epub 2018 Feb 1.
7
Alpha-glucosidase I is required for cellulose biosynthesis and morphogenesis in Arabidopsis.拟南芥中的纤维素生物合成和形态发生需要α-葡萄糖苷酶I。
J Cell Biol. 2002 Mar 18;156(6):1003-13. doi: 10.1083/jcb.200111093.
8
Class I alpha-mannosidases are required for N-glycan processing and root development in Arabidopsis thaliana.I 类α-甘露糖苷酶是拟南芥 N-聚糖加工和根系发育所必需的。
Plant Cell. 2009 Dec;21(12):3850-67. doi: 10.1105/tpc.109.072363. Epub 2009 Dec 18.
9
Glucosidase II and N-glycan mannose content regulate the half-lives of monoglucosylated species in vivo.葡糖苷酶 II 和 N-聚糖甘露糖含量调节体内单糖基化物种的半衰期。
Mol Biol Cell. 2011 Jun 1;22(11):1810-23. doi: 10.1091/mbc.E11-01-0019. Epub 2011 Apr 6.
10
Myrosinases TGG1 and TGG2 from Arabidopsis thaliana contain exclusively oligomannosidic N-glycans.拟南芥的黑芥子酶 TGG1 和 TGG2 仅含有寡甘露糖型 N-聚糖。
Phytochemistry. 2012 Dec;84(21):24-30. doi: 10.1016/j.phytochem.2012.08.023. Epub 2012 Sep 23.
引用本文的文献
1
Insight into broad substrate specificity and synergistic contribution of a fungal α-glucosidase in Chinese Nong-flavor daqu.深入了解中国浓香型大曲中真菌 α-葡萄糖苷酶的广泛底物特异性和协同贡献。
Microb Cell Fact. 2023 Jun 15;22(1):114. doi: 10.1186/s12934-023-02124-z.
2
Characterization of PcSTT3B as a Key Oligosaccharyltransferase Subunit Involved in N-glycosylation and Its Role in Development and Pathogenicity of .PcSTT3B 的鉴定作为一种关键的寡糖基转移酶亚基参与 N-糖基化及其在 和致病性中的作用。
Int J Mol Sci. 2023 Apr 19;24(8):7500. doi: 10.3390/ijms24087500.
3
Processing of the Terminal Alpha-1,2-Linked Mannose Residues From Oligomannosidic -Glycans Is Critical for Proper Root Growth.来自寡甘露糖型聚糖的末端α-1,2-连接甘露糖残基的加工对于根系正常生长至关重要。
Front Plant Sci. 2018 Dec 6;9:1807. doi: 10.3389/fpls.2018.01807. eCollection 2018.
4
A chemical genetic screen reveals that iminosugar inhibitors of plant glucosylceramide synthase inhibit root growth in Arabidopsis and cereals.化学遗传学筛选表明，植物神经酰胺葡萄糖基转移酶的氨基糖抑制剂抑制拟南芥和谷物的根生长。
Sci Rep. 2018 Nov 6;8(1):16421. doi: 10.1038/s41598-018-34749-1.
5
Abrogation of glucosidase I-mediated glycoprotein deglucosylation results in a sick phenotype in fission yeasts: Model for the human MOGS-CDG disorder.糖苷酶 I 介导的糖蛋白去糖基化作用的废除导致裂殖酵母产生病态表型：人类 MOGS-CDG 疾病的模型。
J Biol Chem. 2018 Dec 28;293(52):19957-19973. doi: 10.1074/jbc.RA118.004844. Epub 2018 Nov 2.
6
Protein Quality Control in the Endoplasmic Reticulum of Plants.植物内质网中的蛋白质质量控制。
Annu Rev Plant Biol. 2018 Apr 29;69:147-172. doi: 10.1146/annurev-arplant-042817-040331. Epub 2018 Mar 23.
7
N-Glycomic and Microscopic Subcellular Localization Analyses of NPP1, 2 and 6 Strongly Indicate that trans-Golgi Compartments Participate in the Golgi to Plastid Traffic of Nucleotide Pyrophosphatase/Phosphodiesterases in Rice.对NPP1、2和6进行的N-糖组学及亚细胞微观定位分析有力表明，反式高尔基体区室参与了水稻中核苷酸焦磷酸酶/磷酸二酯酶从高尔基体到质体的转运过程。
Plant Cell Physiol. 2016 Aug;57(8):1610-28. doi: 10.1093/pcp/pcw089. Epub 2016 May 6.
8
Plant protein glycosylation.植物蛋白糖基化
Glycobiology. 2016 Sep;26(9):926-939. doi: 10.1093/glycob/cww023. Epub 2016 Feb 23.
9
Iminosugar inhibitors of carbohydrate-active enzymes that underpin cereal grain germination and endosperm metabolism.支撑谷物种子萌发和胚乳代谢的碳水化合物活性酶的亚氨基糖抑制剂。
Biochem Soc Trans. 2016 Feb;44(1):159-65. doi: 10.1042/BST20150222.
10
Crystal Structure and Functional Analyses of the Lectin Domain of Glucosidase II: Insights into Oligomannose Recognition.葡糖苷酶II凝集素结构域的晶体结构与功能分析：对寡甘露糖识别的见解
Biochemistry. 2015 Jul 7;54(26):4097-111. doi: 10.1021/acs.biochem.5b00256. Epub 2015 Jun 24.
本文引用的文献
1
N-linked glycan recognition and processing: the molecular basis of endoplasmic reticulum quality control.N-连接聚糖识别与加工：内质网质量控制的分子基础
Curr Opin Struct Biol. 2006 Oct;16(5):592-9. doi: 10.1016/j.sbi.2006.08.005. Epub 2006 Aug 30.
2
Yeast GTB1 encodes a subunit of glucosidase II required for glycoprotein processing in the endoplasmic reticulum.酵母GTB1编码在内质网中进行糖蛋白加工所需的葡糖苷酶II的一个亚基。
J Biol Chem. 2006 Mar 10;281(10):6325-33. doi: 10.1074/jbc.M510455200. Epub 2005 Dec 22.
3
Cloning and characterization of the glucosidase II alpha subunit gene of Trichoderma reesei: a frameshift mutation results in the aberrant glycosylation profile of the hypercellulolytic strain Rut-C30.里氏木霉葡糖苷酶IIα亚基基因的克隆与特性分析：一个移码突变导致高纤维素分解菌株Rut-C30的异常糖基化谱。
Appl Environ Microbiol. 2005 Jun;71(6):2910-24. doi: 10.1128/AEM.71.6.2910-2924.2005.
4
Roles of N-linked glycans in the endoplasmic reticulum.N-连接聚糖在内质网中的作用。
Annu Rev Biochem. 2004;73:1019-49. doi: 10.1146/annurev.biochem.73.011303.073752.
5
Generation of Arabidopsis thaliana plants with complex N-glycans lacking beta1,2-linked xylose and core alpha1,3-linked fucose.生成具有复杂N-聚糖的拟南芥植物，这些N-聚糖缺乏β1,2-连接的木糖和核心α1,3-连接的岩藻糖。
FEBS Lett. 2004 Mar 12;561(1-3):132-6. doi: 10.1016/S0014-5793(04)00150-4.
6
Nucleoside diphosphatase and glycosyltransferase activities can localize to different subcellular compartments in Schizosaccharomyces pombe.核苷二磷酸酶和糖基转移酶的活性可以定位于粟酒裂殖酵母的不同亚细胞区室。
J Biol Chem. 2003 Jun 20;278(25):22379-87. doi: 10.1074/jbc.M300892200. Epub 2003 Apr 9.
7
In vitro oligosaccharide synthesis using intact yeast cells that display glycosyltransferases at the cell surface through cell wall-anchored protein Pir.利用完整酵母细胞进行体外寡糖合成，这些酵母细胞通过细胞壁锚定蛋白Pir在细胞表面展示糖基转移酶。
Glycobiology. 2003 Feb;13(2):87-95. doi: 10.1093/glycob/cwg014. Epub 2002 Nov 26.
8
Quality control in the endoplasmic reticulum.内质网中的质量控制
Nat Rev Mol Cell Biol. 2003 Mar;4(3):181-91. doi: 10.1038/nrm1052.
9
The cellulose-deficient Arabidopsis mutant rsw3 is defective in a gene encoding a putative glucosidase II, an enzyme processing N-glycans during ER quality control.缺乏纤维素的拟南芥突变体rsw3在一个编码假定葡糖苷酶II的基因中存在缺陷，该酶在内质网质量控制过程中加工N-聚糖。
Plant J. 2002 Dec;32(6):949-60. doi: 10.1046/j.1365-313x.2002.01483.x.
10
Alpha-glucosidase I is required for cellulose biosynthesis and morphogenesis in Arabidopsis.拟南芥中的纤维素生物合成和形态发生需要α-葡萄糖苷酶I。
J Cell Biol. 2002 Mar 18;156(6):1003-13. doi: 10.1083/jcb.200111093.
Zotero 插件