Pawlik Anna, Janusz Grzegorz, Dębska Iwona, Siwulski Marek, Frąc Magdalena, Rogalski Jerzy
Department of Biochemistry, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland.
Department of Vegetable Crops, Poznań University of Life Sciences, Dąbrowskiego 159, 60-594 Poznań, Poland.
Biomed Res Int. 2015;2015:726149. doi: 10.1155/2015/726149. Epub 2015 Mar 1.
Fourteen Ganoderma lucidum strains from different geographic regions were identified using ITS region sequencing. Based on the sequences obtained, the genomic relationship between the analyzed strains was determined. All G. lucidum strains were also genetically characterized using the AFLP technique. G. lucidum strains included in the analysis displayed an AFLP profile similarity level in the range from 9.6 to 33.9%. Biolog FF MicroPlates were applied to obtain data on utilization of 95 carbon sources and mitochondrial activity. The analysis allowed comparison of functional diversity of the fungal strains. The substrate utilization profiles for the isolates tested revealed a broad variability within the analyzed G. lucidum species and proved to be a good profiling technology for studying the diversity in fungi. Significant differences have been demonstrated in substrate richness values. Interestingly, the analysis of growth and biomass production also differentiated the strains based on the growth rate on the agar and sawdust substrate. In general, the mycelial growth on the sawdust substrate was more balanced and the fastest fungal growth was observed for GRE3 and FCL192.
使用ITS区域测序对来自不同地理区域的14株灵芝菌株进行了鉴定。根据获得的序列，确定了分析菌株之间的基因组关系。所有灵芝菌株还使用AFLP技术进行了遗传特征分析。分析中包括的灵芝菌株显示出AFLP图谱相似性水平在9.6%至33.9%之间。使用Biolog FF微孔板获取关于95种碳源利用和线粒体活性的数据。该分析允许对真菌菌株的功能多样性进行比较。测试分离株的底物利用图谱显示在所分析的灵芝物种内存在广泛的变异性，并被证明是研究真菌多样性的良好分析技术。已证明底物丰富度值存在显著差异。有趣的是，生长和生物量生产分析也根据在琼脂和木屑底物上的生长速率区分了菌株。总体而言，在木屑底物上的菌丝体生长更为平衡，并且观察到GRE3和FCL192的真菌生长最快。
