Ma Lian-Ju, Liu Xin, Zhuo Yu-Juan, Yu Lu-Rong
Chongqing Medical University, Chongqing 400016, China.
Zhong Yao Cai. 2008 Jul;31(7):1065-7.
To establish a convenient, practical and environmental method for extracting and isolating shikimic acid.
The content of shikimic acid was measured by RP-ion-pair HPLC, the effects of different pH, temperature, sample concentration and eluate concentration on 717 anion exchange resin were studied.
The yield of shikimic acid was 96.52% on the condition of 22 degrees C, pH > 6.5, 7.5 mg /ml sample concentration and 0.03 mol/L HCl eluting.
This method is feasible and suitable for the extraction and isolation of shikimic acid.
建立一种简便、实用且环保的莽草酸提取与分离方法。
采用反相离子对高效液相色谱法测定莽草酸含量，研究不同pH值、温度、样品浓度及洗脱液浓度对717阴离子交换树脂的影响。
在22℃、pH>6.5、样品浓度7.5mg/ml及0.03mol/L盐酸洗脱条件下，莽草酸得率为96.52%。
该方法可行，适用于莽草酸的提取与分离。
