Wang X Q, Guo Y J, Yang C S
Beijing University of Chinese Medicine, Beijing 100029, China.
Zhongguo Zhong Yao Za Zhi. 2001 Jul;26(7):447-9.
To establish the determination method of Shikimic acid.
Using HPLC method as the determination method. The separation was performed in a SiO2-NH2 column with a mobile phase of Acetonitrile-2% H3PO4 water solution (95:5); The sample wavelength was 213 nm, reference wavelength 300 nm.
The average collection was 98.5%, RSD 1.67% (n = 5).
This method is suitable for the determination of Shkimic acid in herb medicines and preparation containing shikimic acid.
建立莽草酸的测定方法。
采用高效液相色谱法作为测定方法。在硅胶 - 氨基柱上进行分离，流动相为乙腈 - 2%磷酸水溶液（95:5）；样品波长为213nm，参比波长为300nm。
平均回收率为98.5%，相对标准偏差为1.67%（n = 5）。
该方法适用于含莽草酸的中药材及制剂中莽草酸的测定。
