Fagrell Tobias G, Dietz Wolfram, Lingström Peter, Steiniger Frank, Norén Jörgen G
' Specialist Clinic of Pedodontics, Sahlgrenska University Hospital Mölndal, Sweden.
Swed Dent J. 2008;32(3):139-47.
Ozone treatment has been presented and discussed in the literature, as one of the "new" ways to treat dental caries. The aim of this paper was to study the in vitro effect of ozone on some common oral microorganisms related to dental caries using scanning electron microscopy (SEM). The effect of ozone was tested on three different strains of mutans streptococci and one Lactobacillus strain. After exposure of bacteria to ozone treatment for 0 to 60 sec, cultivation on different chair side strips and agar plates took place. Preparation and performance of scanning electron analyses in a field emission scanning electron microscope at 10 kV was then carried out. It was found that gaseous ozone treatment for 20 seconds or more was effective to kill the different microorganisms in vitro. Treatment of 20, 40 and 60 seconds of ozone prevented the bacteria to grow on the different media. Treatment times shorter than 20 seconds resulted in varying results with a limited effect on bacterial growth for treatments of 5-10 sec, respectively. The difference between ozone-treated and untreated specimens was macroscopically readily discernable. None of the strains treated with ozone for 60 seconds showed any bacterial growth. Only samples with untreated bacteria could be found in the SEM analyses in form of large and high colonies. This study presents a clear result of the bactericide effect of ozon (in vitro) on four different strains of bacteria associated with dental caries.
臭氧治疗作为治疗龋齿的“新”方法之一，已在文献中有所介绍和讨论。本文的目的是使用扫描电子显微镜（SEM）研究臭氧对一些与龋齿相关的常见口腔微生物的体外作用。测试了臭氧对三种不同变形链球菌菌株和一种乳酸杆菌菌株的作用。将细菌暴露于臭氧处理0至60秒后，在不同的椅旁试纸条和琼脂平板上进行培养。然后在10 kV的场发射扫描电子显微镜中进行扫描电子分析的制备和操作。结果发现，气态臭氧处理20秒或更长时间可有效在体外杀死不同的微生物。20、40和60秒的臭氧处理可防止细菌在不同培养基上生长。短于20秒的处理时间导致结果各异，5至10秒的处理对细菌生长的影响有限。臭氧处理和未处理标本之间的差异在宏观上很容易辨别。用臭氧处理60秒的菌株均未显示任何细菌生长。在扫描电子显微镜分析中，仅能发现未处理细菌的样本呈现为大的高菌落形式。本研究明确呈现了臭氧（体外）对四种与龋齿相关的不同细菌菌株的杀菌作用结果。
