Dukić Walter, Jurić Hrvoje, Andrasević Arjana Tambić, Kovacević Visnja, Dukić Olga Lulić, Delija Barbara
University of Zagreb, School of Dental Medicine, Department of Pediatric Dentistry, Zagreb, Croatia.
Coll Antropol. 2013 Mar;37(1):109-13.
The aim of this study is to analyze ozone impact on some cariogenic bacteria in ex vivo and in vitro conditions. The in vitro part of study inoculated dentine with strains of Streptococcus mutans ATCC 33402 and Lactobacillus paracasei ATCC 11974 bacteria. Samples of dentine before and after 40s ozone treatment were collected and anaerobically incubated. Samples of cariogenic dentine (N = 24) were collected from permanent molars within the ex vivo segment of the study, prior and after 40s ozone treatment and a number of colonies were counted after incubation. For the in vitro part of study, results have shown a statistically significant average value of reduction of Streptococcus mutans ATCC 33402 and Lactobacillus paracasei ATCC 11974 prior and after ozone treatment (p < 0.001). The ex vivo segment of the study has also demonstrated a statistically significant difference in the number of bacteria prior and after ozone implementation (p < 0.001). Gaseous ozone demonstrated a strong antimicrobial effect on cariogenic bacteria in both in vitro and ex vivo conditions and it can be used as an adjuvant in caries therapy.
本研究的目的是分析臭氧在体外和体内条件下对一些致龋菌的影响。研究的体外部分用变形链球菌ATCC 33402菌株和副干酪乳杆菌ATCC 11974细菌接种牙本质。收集40秒臭氧处理前后的牙本质样本并进行厌氧培养。在研究的体内部分，从恒磨牙收集致龋牙本质样本（N = 24），在40秒臭氧处理前后进行收集，并在培养后计数菌落数量。对于研究的体外部分，结果显示在臭氧处理前后，变形链球菌ATCC 33402和副干酪乳杆菌ATCC 11974的平均减少值具有统计学意义（p < 0.001）。研究的体内部分也表明，在实施臭氧前后细菌数量存在统计学显著差异（p < 0.001）。气态臭氧在体外和体内条件下均对致龋菌表现出强烈的抗菌作用，可作为龋齿治疗的辅助手段。
