Wollin A, Navert H
Anal Biochem. 1985 Feb 15;145(1):73-9. doi: 10.1016/0003-2697(85)90328-8.
A method which measures histamine, its basic metabolites, and some analogs in biological materials is described. The procedure consists of ion-exchange chromatography and chromatography on silicic acid for isolation and purification, and gas chromatography for identification and quantitation. The isolated compounds are prepared for gas chromatography by double derivatization with heptafluorobutyric anhydride and acetic anhydride. One of the synthetic histamine analogs, 2-methylhistamine, is used as an internal standard. The metabolites can be quantitated at a level as low as 1.5 nmol/100 microliters final concentration. The method is adaptable to a variety of applications, with good reproducibility and sensitivity. A number of different biological samples have been analyzed.
本文描述了一种测定生物材料中组胺及其基本代谢产物和一些类似物的方法。该方法包括离子交换色谱法和硅酸柱色谱法用于分离和纯化，以及气相色谱法用于鉴定和定量。分离出的化合物通过用七氟丁酸酐和乙酸酐进行双重衍生化来制备用于气相色谱分析。合成组胺类似物之一2-甲基组胺用作内标。代谢产物的定量下限可达最终浓度1.5纳摩尔/100微升。该方法适用于多种应用，具有良好的重现性和灵敏度。已对多种不同的生物样品进行了分析。
