Mendiratta D K, Kaur H, Deotale V, Thamke D C, Narang R, Narang P
Department of Microbiology, Mahatma Gandhi Institute of Medical Sciences, Sevagram, Maharashtra, India.
Indian J Med Microbiol. 2008 Oct-Dec;26(4):369-71. doi: 10.4103/0255-0857.43582.
Considering the emergence of high level aminoglycoside resistance (HLAR) in enterococci this study was undertaken to determine their status in a rural setting. HLAR by disc diffusion and agar dilution, beta lactamase by nitrocefin disc and vancomycin resistance by agar dilution was determined in 150 enterococcal isolates, as per NCCLS guidelines. Only two species, Enterococcus faecalis (85.5%) and Enterococcus faecium (14.7%) were recovered, mostly from blood. Forty six percent showed HLAR. Multi drug resistance and concomitant resistance of HLAR strains to beta lactams were quite high. None showed beta lactamase activity or vancomycin resistance.
鉴于肠球菌中高水平氨基糖苷类耐药性（HLAR）的出现，开展了本研究以确定其在农村地区的情况。按照美国国家临床实验室标准委员会（NCCLS）指南，对150株肠球菌分离株进行了纸片扩散法和琼脂稀释法检测HLAR、硝基头孢菌素纸片法检测β-内酰胺酶以及琼脂稀释法检测万古霉素耐药性。仅分离出两种菌，粪肠球菌（85.5%）和屎肠球菌（14.7%），大多来自血液。46%的菌株表现出HLAR。HLAR菌株的多重耐药性以及对β-内酰胺类药物的伴随耐药性相当高。未发现有β-内酰胺酶活性或万古霉素耐药性的菌株。
