Zimatkin S M, Baraban O V, Emel'yanchik S V
Department of Histology, Cytology, and Embryology, Grodno State Medical University, Grodno, Belarus.
Neurosci Behav Physiol. 2008 Nov;38(9):907-11. doi: 10.1007/s11055-008-9069-3. Epub 2008 Oct 31.
The aim of the present work was to evaluate structural and metabolic changes in histaminergic neurons in hypothalamic nucleus E2 in rats in conditions of complete external drainage of bile. Studies were performed on male Wistar rats (n = 45). Controls consisted of animals subjected to sham surgery with preservation of physiological bile flow throughout the experiment. Quantitative histological and histochemical methods were used. Serial frontal cryostat sections cut from the posterior hypothalamus were used for detection of the activity of the following enzymes: monoamine oxidase B, succinate dehydrogenase, NADH dehydrogenase, NADPH dehydrogenase, glucose-6-phosphate dehydrogenase, lactate dehydrogenase, and acid phosphatase. Morphological studies of histaminergic neurons were performed on preparations stained with thionine. These studies showed that complete external drainage of bile led to transient size reductions and rounding of cell perikarya. Metabolic changes were seen within a day of bile loss and subsequently progressed. All energy metabolic pathways were suppressed and acid phosphatase activity was increased on day 5.
本研究的目的是评估在胆汁完全外引流的情况下，大鼠下丘脑E2核中组胺能神经元的结构和代谢变化。实验选用雄性Wistar大鼠（n = 45）。对照组为在整个实验过程中接受假手术且生理胆汁流动得以保留的动物。采用了定量组织学和组织化学方法。从下丘脑后部切取的连续额状低温恒冷切片用于检测以下酶的活性：单胺氧化酶B、琥珀酸脱氢酶、NADH脱氢酶、NADPH脱氢酶、葡萄糖-6-磷酸脱氢酶、乳酸脱氢酶和酸性磷酸酶。对硫堇染色的标本进行组胺能神经元的形态学研究。这些研究表明，胆汁完全外引流导致细胞周体暂时缩小并变圆。胆汁流失一天内即可观察到代谢变化，随后变化持续发展。第5天时，所有能量代谢途径均受到抑制，酸性磷酸酶活性增加。
