Zimatkin S M, Kuznetsova V B, Strik O N
Department of Histology, Cytology, and Embryology and Central Science Research Laboratory, Grodno State Medical University, Belarus.
Neurosci Behav Physiol. 2006 Jun;36(5):467-71. doi: 10.1007/s11055-006-0041-9.
We report here a study addressing the spatial organization, densities, numbers, sizes, and shapes of histaminergic neurons in the rat hypothalamus. Studies were performed on 50 rats using histochemical and morphometric methods, computer image analysis, and three-dimensional reconstruction of the histaminergic nuclei of the hypothalamus. The results demonstrated that the total bilateral volume of the histaminergic nuclei of the rat brain amounts to 0.5 mm(3): the E2 nucleus occupies 40% of the total volume, E4 occupies 35%, E3 occupies 13%, E5 occupies 9%, and E1 occupies 3%. The distribution density of monoamine oxidase B neurons in the histaminergic nuclei of the rat hypothalamus decreased in the order E1 > E3 > E2 (the "compact" nuclei) > E4 (an "intermediate" density nucleus) > E5 (the "diffuse part"). The mean number of histaminergic neurons in the rat hypothalamus was 37200 +/- 2800; the E2 nucleus contained 54% of these cells, E3 contained 23%, E4 contained 6%, E1 contained 7%, and E5 contained 0.32%. The E1-E3 nuclei were dominated by small and intermediate-sized neurons, round in shape, and the E5 nucleus was dominated by intermediate and large neurons, fusiform in shape. A subpopulation of giant histaminergic neurons was detected in the E4-E5 nuclei.
我们在此报告一项关于大鼠下丘脑组胺能神经元的空间组织、密度、数量、大小和形状的研究。使用组织化学和形态计量学方法、计算机图像分析以及下丘脑组胺能核的三维重建技术，对50只大鼠进行了研究。结果表明，大鼠脑内组胺能核的双侧总体积为0.5立方毫米：E2核占总体积的40%，E4占35%，E3占13%，E5占9%，E1占3%。大鼠下丘脑组胺能核中单胺氧化酶B神经元的分布密度依次为E1>E3>E2（“致密”核）>E4（“中等密度”核）>E5（“弥散部分”）。大鼠下丘脑组胺能神经元的平均数量为37200±2800；E2核包含这些细胞的54%，E3包含23%，E4包含6%，E1包含7%，E5包含0.32%。E1-E3核以小和中等大小的圆形神经元为主，E5核以中等和大的梭形神经元为主。在E4-E5核中检测到一群巨大的组胺能神经元。
