Kilikian B V, Afonso L C, Souza T F C, Ferreira R G, Pinheiro I R
Departamento de Engenharia Química Universidade de São Paulo São PauloSP Brazil.
Departamento de Engenharia Rural Centro de Ciências Agrárias Universidade Federal do Espírito Santo GuararemaES Brazil.
Braz J Microbiol. 2014 Apr 11;45(1):279-86. doi: 10.1590/S1517-83822014005000028. eCollection 2014.
Cellulase production was evaluated in two reference strains (T. reesei Rut-C30 and T. reesei QM9414), two strains isolated from a sugarcane cultivation area (Trichoderma sp. IPT778 and T. harzianum rifai IPT821) and one strain isolated in a program for biodiversity preservation in São Paulo state (Myceliophthora thermophila M77). Solid state cultures were performed using sugarcane bagasse (C), wheat bran (W) and/or soybean bran (S). The highest FPA was 10.6 U/gdm for M77 in SC (10:90) at 80% moisture, which was 4.4 times higher than production in pure W. C was a strong inducer of cellulase production, given that the production level of 6.1 U/gdm in WC (40:60) was 2.5 times higher than in pure W for strain M77; T. reesei Rut-C30 did not respond as strongly with about 1.6-fold surplus production. S advantageously replaced W, as the surplus production on SC (20:80) was 2.3 times relative to WC (20:80) for M77.
在两种参考菌株（里氏木霉Rut-C30和里氏木霉QM9414）、两种从甘蔗种植区分离得到的菌株（木霉属IPT778和哈茨木霉rifai IPT821）以及一种在圣保罗州生物多样性保护项目中分离得到的菌株（嗜热毁丝霉M77）中评估了纤维素酶的产生情况。使用甘蔗渣（C）、麦麸（W）和/或大豆麸（S）进行固态培养。在80%湿度下，M77在SC（10:90）中的最高FPA为10.6 U/gdm，这比在纯W中的产量高4.4倍。C是纤维素酶产生的强诱导剂，因为对于菌株M77，WC（40:60）中6.1 U/gdm的产量水平比纯W中的产量高2.5倍；里氏木霉Rut-C30的反应没有那么强烈，产量仅过剩约1.6倍。S有利地替代了W，因为对于M77，SC（20:80）上的过剩产量相对于WC（20:80）是2.3倍。
