Darabzadeh Nazanin, Hamidi-Esfahani Zohreh, Hejazi Parisa
Department of Food Science and Industry Faculty of Agriculture Tarbiat Modares University Tehran Iran.
School of Chemical, Petroleum and Gas Engineering Iran University of Science and Technology Tehran Iran.
Food Sci Nutr. 2018 Oct 29;7(2):572-578. doi: 10.1002/fsn3.852. eCollection 2019 Feb.
Nowadays, the use of agricultural by-products, as the cheap substrate for the production of value-added products, is of high interest for the researchers and practitioners. Cellulase is a relatively expensive and a very important industrial enzyme where in this study was produced form rice by-products under solid-state fermentation. A new mutant of was used for cellulase production. The effective variables were initially screened by "Plackett and Burman design." Afterward, the main variables including moisture content, P source, incubation temperature, and incubation time were optimized by "one factor at a time design." Finally, the resulting variables including 74% for moisture content, 2 g/L for K HPO , 30°C of incubation temperature, and 4 days of incubation time were reported as the ultimate optimal condition for cellulase production.
如今，将农业副产品用作生产增值产品的廉价底物，受到了研究人员和从业者的高度关注。纤维素酶是一种相对昂贵但非常重要的工业酶，在本研究中通过固态发酵从大米副产品中生产。使用一种新的突变体来生产纤维素酶。有效变量最初通过“Plackett和Burman设计”进行筛选。之后，通过“一次一个因素设计”对包括水分含量、磷源、培养温度和培养时间在内的主要变量进行了优化。最后，报告了水分含量74%、磷酸氢二钾2 g/L、培养温度30°C和培养时间4天等所得变量，作为纤维素酶生产的最终最佳条件。
