一种通过改良的锌荧光传感器定量生物锌的新策略。
New strategy for quantifying biological zinc by a modified zinpyr fluorescence sensor.
作者信息
Zhang Xiao-an, Hayes Dugan, Smith Sarah J, Friedle Simone, Lippard Stephen J
机构信息
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
出版信息
J Am Chem Soc. 2008 Nov 26;130(47):15788-9. doi: 10.1021/ja807156b.
Abstract
Substitution of one pyridine by pyrazine in each DPA appendage of ZP1 leads to a new zinc sensor, ZPP1, with a modified background fluorescence and zinc affinity. ZPP1 exhibits a two-step zinc response during fluorescence titrations, which leads to a new method for zinc quantification. The ability of ZPP1 to image and quantify zinc was demonstrated in pancreatic Min6 cells.
摘要
在ZP1的每个DPA附属物中用吡嗪取代一个吡啶会产生一种新的锌传感器ZPP1，其背景荧光和锌亲和力都有所改变。在荧光滴定过程中，ZPP1呈现出两步锌响应，这导致了一种新的锌定量方法。ZPP1对胰腺Min6细胞中的锌进行成像和定量的能力得到了证实。
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