Gudmundsdóttir S, Magnadóttir B, Björnsdóttir B, Arnadóttir H, Gudmundsdóttir B K
Department of Fish Diseases, Institute for Experimental Pathology, University of Iceland, Reykjavík, Iceland.
Fish Shellfish Immunol. 2009 Apr;26(4):619-24. doi: 10.1016/j.fsi.2008.09.017. Epub 2008 Oct 14.
The purpose of the present study was to study specific and natural antibody levels in individual cod juveniles before and after being vaccinated against Vibrio anguillarum. Different vaccine preparations and vaccination regimes, i.e. bathing, dipping, i.p. injection or combination of treatments were employed and the performance of different groups to bath challenge by the bacterium tested. Antibody responses to V. anguillarum antigens in groups vaccinated by bathing and/or dipping were negligible, while responses were observed in i.p. injected fish. Fish receiving i.p. injection in addition to bathing, showed significant antibody response. Both groups showed increased levels of natural antibodies while levels were low in other groups. Fish bathed or dipped showed higher mortality when challenged than untreated fish, while fish that received a second vaccination showed the best protection. It was not ascertained whether there is a long term difference between the effects of immersion versus i.p. injection as a booster method. Levels of antibodies against V. anguillarum antigens or natural antibodies in groups with the lowest mortalities show that neither could have been used to predict protection given by the vaccines tested.
本研究的目的是研究单个鳕鱼幼鱼在接种鳗弧菌疫苗前后的特异性抗体和天然抗体水平。采用了不同的疫苗制剂和接种方式，即浸浴、浸泡、腹腔注射或多种处理方式的组合，并测试了不同组在细菌浸浴攻击下的表现。通过浸浴和/或浸泡接种疫苗的组对鳗弧菌抗原的抗体反应可忽略不计，而腹腔注射的鱼则有抗体反应。除浸浴外还接受腹腔注射的鱼表现出显著的抗体反应。两组的天然抗体水平均有所升高，而其他组的水平较低。浸浴或浸泡的鱼在受到攻击时比未处理的鱼死亡率更高，而接受二次接种的鱼表现出最佳的保护效果。作为加强免疫方法，浸浴与腹腔注射的效果是否存在长期差异尚未确定。死亡率最低的组中针对鳗弧菌抗原的抗体水平或天然抗体水平表明，两者均不能用于预测所测试疫苗提供的保护作用。
