Gottlieb C, Bygdeman M, Thyberg P, Hellman B, Rigler R
Department of Obstetrics and Gynecology, Karolinska Hospital, Stockholm, Sweden.
Andrologia. 1991 Jan-Feb;23(1):1-5. doi: 10.1111/j.1439-0272.1991.tb02480.x.
A new method which allows separation of the rotational and translational components of human sperm motility, based on the angular dependence of the dynamic laser light scattering (DLS) has been developed. The technique was used in a clinical study and was compared with an independent evaluation by video micrography. A good correlation was found between the two techniques when applied on different semen samples (r = 0.90; P less than 0.01 and r = 0.96; P less than 0.01 for rotation and translation, respectively) and when applied on the same semen sample at different temperatures. The rapid evaluation of these parameters using DLS technique opens the possibility to study large number of semen samples under physiological and pathological conditions at a lower cost.
基于动态激光散射（DLS）的角度依赖性，已开发出一种可分离人类精子运动旋转和平移成分的新方法。该技术用于一项临床研究，并与视频显微镜的独立评估进行了比较。当应用于不同精液样本时（旋转和平移的相关系数分别为r = 0.90；P < 0.01和r = 0.96；P < 0.01）以及当在不同温度下应用于同一精液样本时，两种技术之间发现了良好的相关性。使用DLS技术快速评估这些参数，为以较低成本在生理和病理条件下研究大量精液样本开辟了可能性。
