Jouannet P, Volochine B, Deguent P, Serres C, David G
Andrologia. 1977 Jan-Mar;9(1):36-49. doi: 10.1111/j.1439-0272.1977.tb01257.x.
In this paper are presented the results obtained by the light scattering method of systematic study of different parameters characterizing spermatozoa motility in a series of human sperms, one hour and five hours after ejaculation. These results are relative to the concentration of spermatozoa, the percentage and the number of motile spermatozoa, the characteristic velocity and the velocity distributions. All these parameters are discussed one by one, the advantages (objectivity, small quantity of not diluted sperm, short time needed for measurements) and the limits of the light scattering method being pointed out. A comparison is made with results obtained by conventional methods. The fineness of this study shows that this method can be used with success in fundamental research as well as in a routine functional sperm study, allowing an objective and precise analysis of motility in a given population as a complex factor.
本文介绍了通过光散射法对一系列人类精子射精后1小时和5小时不同精子活力参数进行系统研究所得的结果。这些结果涉及精子浓度、活动精子的百分比和数量、特征速度以及速度分布。逐一讨论了所有这些参数，并指出了光散射法的优点（客观性、所需未稀释精子量少、测量所需时间短）和局限性。与传统方法所得结果进行了比较。这项研究的精细程度表明，该方法可成功用于基础研究以及常规精子功能研究，能够对特定群体中的活力这一复杂因素进行客观、精确的分析。
