Mohamed M K, Lee W I, Mottet N K, Burbacher T M
J Androl. 1986 Jan-Feb;7(1):11-5. doi: 10.1002/j.1939-4640.1986.tb00858.x.
An in vitro study was designed using the laser light-scattering technique to obtain further information on the dose-effect relationship of methylmercury on sperm motility. The technique provided a quantitative evaluation of sperm swimming speed. Semen samples were collected from normal male Macaca fascicularis monkeys by anal electroejaculation. Methylmercury was added to aliquots of sperm suspensions in BWW medium in doses of 10, 5, 2, and 1 ppm. After 3 hours, the relative speed was 35%, 59%, 69%, and 92% of the corresponding controls at doses of 10, 5, 2, and 1 ppm, respectively. The percentage of motile spermatozoa decreased significantly at 10 ppm. By microscopic observation abnormal motility was detected at 5 and 10 ppm, especially after 20 to 40 minutes. Head movement increased from side to side, and many spermatozoa developed coiled tails. The technique proved useful for defining the dose-effect relationship of methylmercury and sperm swimming speed.
设计了一项体外研究，采用激光散射技术来获取更多关于甲基汞对精子活力剂量效应关系的信息。该技术可对精子游动速度进行定量评估。通过肛门电刺激射精从正常雄性食蟹猴采集精液样本。将甲基汞以10、5、2和1 ppm的剂量添加到BWW培养基中的精子悬浮液等分试样中。3小时后，在10、5、2和1 ppm剂量下，相对速度分别为相应对照组的35%、59%、69%和92%。在10 ppm时，活动精子的百分比显著下降。通过显微镜观察，在5和10 ppm时检测到异常活力，尤其是在20至40分钟后。头部运动从一侧到另一侧增加，许多精子出现尾部卷曲。该技术被证明有助于确定甲基汞与精子游动速度的剂量效应关系。
