Neumann Eric J, Grinberg Alex, Bonistalli Kathryn N, Mack Hamish J, Lehrbach Philip R, Gibson Nicole
Institute of Veterinary, Animal and Biomedical Sciences, College of Sciences, Massey University, Tennent Drive, Palmerston North 4442, New Zealand.
Vet Microbiol. 2009 Mar 30;135(3-4):297-303. doi: 10.1016/j.vetmic.2008.09.059. Epub 2008 Sep 21.
Infection with Erysipelothrix rhusiopathiae has a significant economic impact on pig production systems worldwide. Both inactivated and attenuated vaccines are available to prevent development of clinical signs of swine erysipelas. The ability of a live attenuated E. rhusiopathiae strain to become persistently established in pigs after intranasal exposure and its potential to cause clinical signs consistent with swine erysipelas after being administered directly into the nasopharynx of healthy pigs was evaluated. Five, E. rhusiopathiae-negative pigs were vaccinated by deep intranasal inoculation then followed for 14 days. Nasal swabs were collected daily for 5 days and clinical observations were made daily for 14 days post-vaccination. Nasal swabs were cultured for E. rhusiopathiae with the intent of back-passaging any recovered organisms into subsequent replicates. No organism was recovered from nasal swabs in the first vaccination replicate. A second replicate including 10 pigs was initiated and followed in an identical manner to that described above. Again, no E. rhusiopathiae was recovered from any pigs. No pigs in either replicate showed any signs of clinical swine erysipelas. The live attenuated E. rhusiopathiae strain evaluated in this study did not appear to become persistently established in pigs post-vaccination, did not cause any local or systemic signs consistent with swine erysipelas, and was therefore unlikely to revert to a virulent state when used in a field setting.
感染猪红斑丹毒丝菌对全球养猪生产系统具有重大经济影响。目前有灭活疫苗和减毒活疫苗可用于预防猪丹毒临床症状的出现。本研究评估了一种猪红斑丹毒丝菌减毒活菌株经鼻内接种后在猪体内持续定植的能力，以及将其直接接种到健康猪的鼻咽部后引发与猪丹毒一致的临床症状的可能性。五头猪红斑丹毒丝菌阴性猪通过深部鼻内接种进行疫苗接种，随后观察14天。接种疫苗后连续5天每天采集鼻拭子，连续14天每天进行临床观察。对鼻拭子进行猪红斑丹毒丝菌培养，目的是将任何分离出的菌株回传至后续重复试验中。在第一次疫苗接种重复试验中，鼻拭子未分离出任何菌株。启动了第二次重复试验，包括10头猪，并采用与上述相同的方式进行观察。同样，任何猪均未分离出猪红斑丹毒丝菌。两个重复试验中的任何猪均未表现出猪丹毒的任何临床症状。本研究中评估的猪红斑丹毒丝菌减毒活菌株在接种疫苗后似乎未在猪体内持续定植，未引起任何与猪丹毒一致的局部或全身症状，因此在田间使用时不太可能恢复为毒力状态。
