SecB介导的ATP水解与多肽转运之间耦合的最大效率需要两个SecA原聚体。
Maximal efficiency of coupling between ATP hydrolysis and translocation of polypeptides mediated by SecB requires two protomers of SecA.
作者信息
Mao Chunfeng, Hardy Simon J S, Randall Linda L
机构信息
Department of Biochemistry, University of Missouri, Columbia, Missouri 65211, USA.
出版信息
J Bacteriol. 2009 Feb;191(3):978-84. doi: 10.1128/JB.01321-08. Epub 2008 Oct 31.
Abstract
SecA is the ATPase that provides energy for translocation of precursor polypeptides through the SecYEG translocon in Escherichia coli during protein export. We showed previously that when SecA receives the precursor from SecB, the ternary complex is fully active only when two protomers of SecA are bound. Here we used variants of SecA and of SecB that populate complexes containing two protomers of SecA to different degrees to examine both the hydrolysis of ATP and the translocation of polypeptides. We conclude that the low activity of the complexes with only one protomer is the result of a low efficiency of coupling between ATP hydrolysis and translocation.
摘要
SecA是一种ATP酶，在蛋白质输出过程中，它为大肠杆菌中前体多肽通过SecYEG转运体的转运提供能量。我们之前表明，当SecA从SecB接收前体时，只有当两个SecA原体结合时，三元复合物才具有完全活性。在这里，我们使用了SecA和SecB的变体，它们以不同程度形成包含两个SecA原体的复合物，以研究ATP的水解和多肽的转运。我们得出结论，只有一个原体的复合物活性较低是ATP水解与转运之间偶联效率较低的结果。
相似文献
1
Maximal efficiency of coupling between ATP hydrolysis and translocation of polypeptides mediated by SecB requires two protomers of SecA.SecB介导的ATP水解与多肽转运之间耦合的最大效率需要两个SecA原聚体。
J Bacteriol. 2009 Feb;191(3):978-84. doi: 10.1128/JB.01321-08. Epub 2008 Oct 31.
2
The basis of asymmetry in the SecA:SecB complex.SecA:SecB复合物中不对称性的基础。
J Mol Biol. 2015 Feb 27;427(4):887-900. doi: 10.1016/j.jmb.2014.12.008. Epub 2014 Dec 19.
3
A molecular switch in SecA protein couples ATP hydrolysis to protein translocation.SecA蛋白中的一种分子开关将ATP水解与蛋白质转运偶联起来。
Mol Microbiol. 1999 Dec;34(5):1133-45. doi: 10.1046/j.1365-2958.1999.01686.x.
4
SecB modulates the nucleotide-bound state of SecA and stimulates ATPase activity.SecB调节SecA的核苷酸结合状态并刺激ATP酶活性。
Biochemistry. 2002 Apr 23;41(16):5325-32. doi: 10.1021/bi025639p.
5
Asymmetric binding between SecA and SecB two symmetric proteins: implications for function in export.SecA和SecB这两种对称蛋白质之间的不对称结合：对输出功能的影响
J Mol Biol. 2005 Apr 29;348(2):479-89. doi: 10.1016/j.jmb.2005.02.036.
6
Quaternary dynamics of the SecA motor drive translocase catalysis.SecA 马达驱动易位子催化的四级动力学。
Mol Cell. 2013 Dec 12;52(5):655-66. doi: 10.1016/j.molcel.2013.10.036.
7
Coassembly of SecYEG and SecA Fully Restores the Properties of the Native Translocon.SecYEG 和 SecA 的共组装完全恢复了天然易位子的性质。
J Bacteriol. 2018 Dec 7;201(1). doi: 10.1128/JB.00493-18. Print 2019 Jan 1.
8
The molecular chaperone SecB is released from the carboxy-terminus of SecA during initiation of precursor protein translocation.在前体蛋白转运起始过程中，分子伴侣SecB从SecA的羧基末端释放出来。
EMBO J. 1997 Oct 15;16(20):6105-13. doi: 10.1093/emboj/16.20.6105.
9
Electrophysiological studies in Xenopus oocytes for the opening of Escherichia coli SecA-dependent protein-conducting channels.非洲爪蟾卵母细胞中关于大肠杆菌SecA依赖性蛋白质传导通道开放的电生理研究。
J Membr Biol. 2006;214(2):103-13. doi: 10.1007/s00232-006-0079-1. Epub 2007 May 25.
10
Penetration into membrane of amino-terminal region of SecA when associated with SecYEG in active complexes.与 SecYEG 结合在活性复合物中时，SecA 的氨基末端区域穿透膜。
Protein Sci. 2018 Mar;27(3):681-691. doi: 10.1002/pro.3362. Epub 2018 Feb 5.
引用本文的文献
1
A unifying mechanism for protein transport through the core bacterial Sec machinery.一种统一的机制，用于通过核心细菌 Sec 机械进行蛋白质运输。
Open Biol. 2023 Aug;13(8):230166. doi: 10.1098/rsob.230166. Epub 2023 Aug 30.
2
Cotranslational folding of alkaline phosphatase in the periplasm of Escherichia coli.碱性磷酸酶在大肠杆菌周质中的共翻译折叠。
Protein Sci. 2020 Oct;29(10):2028-2037. doi: 10.1002/pro.3927. Epub 2020 Aug 24.
3
Protein Translocation Activity in Surface-Supported Lipid Bilayers.表面支撑脂质双层中的蛋白转运活性。
Langmuir. 2019 Sep 17;35(37):12246-12256. doi: 10.1021/acs.langmuir.9b01928. Epub 2019 Sep 6.
4
Direct visualization of the Sec translocase engaging precursor proteins in lipid bilayers.直接观察 Sec 转运蛋白在脂质双层中与前体蛋白结合。
Sci Adv. 2019 Jun 12;5(6):eaav9404. doi: 10.1126/sciadv.aav9404. eCollection 2019 Jun.
5
Single-molecule observation of nucleotide induced conformational changes in basal SecA-ATP hydrolysis.单分子观测核苷酸诱导基础态 SecA-ATP 水解的构象变化。
Sci Adv. 2018 Oct 24;4(10):eaat8797. doi: 10.1126/sciadv.aat8797. eCollection 2018 Oct.
6
Coassembly of SecYEG and SecA Fully Restores the Properties of the Native Translocon.SecYEG 和 SecA 的共组装完全恢复了天然易位子的性质。
J Bacteriol. 2018 Dec 7;201(1). doi: 10.1128/JB.00493-18. Print 2019 Jan 1.
7
SecA inhibitors as potential antimicrobial agents: differential actions on SecA-only and SecA-SecYEG protein-conducting channels.作为潜在抗菌剂的SecA抑制剂：对仅含SecA和SecA-SecYEG蛋白质传导通道的不同作用。
FEMS Microbiol Lett. 2018 Aug 1;365(15). doi: 10.1093/femsle/fny145.
8
The Sec System: Protein Export in .Sec系统：蛋白质输出……（原文此处不完整）
EcoSal Plus. 2017 Nov;7(2). doi: 10.1128/ecosalplus.ESP-0002-2017.
9
SecA: a potential antimicrobial target.SecA：一种潜在的抗菌靶点。
Future Med Chem. 2015;7(8):989-1007. doi: 10.4155/fmc.15.42.
10
Large-scale evolutionary analyses on SecB subunits of bacterial sec system.细菌Sec系统SecB亚基的大规模进化分析
PLoS One. 2015 Mar 16;10(3):e0120417. doi: 10.1371/journal.pone.0120417. eCollection 2015.
本文引用的文献
1
Full-length Escherichia coli SecA dimerizes in a closed conformation in solution as determined by cryo-electron microscopy.通过冷冻电子显微镜确定，全长大肠杆菌SecA在溶液中以封闭构象二聚化。
J Biol Chem. 2008 Oct 24;283(43):28783-7. doi: 10.1074/jbc.C800160200. Epub 2008 Sep 4.
2
Reexamination of the role of the amino terminus of SecA in promoting its dimerization and functional state.重新审视SecA氨基末端在促进其二聚化和功能状态方面的作用。
J Bacteriol. 2008 Nov;190(21):7302-7. doi: 10.1128/JB.00593-08. Epub 2008 Aug 22.
3
SecA, the motor of the secretion machine, binds diverse partners on one interactive surface.SecA作为分泌机器的动力蛋白，在一个相互作用表面上结合多种伙伴。
J Mol Biol. 2008 Sep 26;382(1):74-87. doi: 10.1016/j.jmb.2008.06.049. Epub 2008 Jun 24.
4
Bacterial protein secretion through the translocase nanomachine.细菌蛋白通过转位酶纳米机器的分泌。
Nat Rev Microbiol. 2007 Nov;5(11):839-51. doi: 10.1038/nrmicro1771.
5
Protein translocation is mediated by oligomers of the SecY complex with one SecY copy forming the channel.蛋白质转运由SecY复合体的寡聚体介导，其中一个SecY拷贝形成通道。
Cell. 2007 Apr 6;129(1):97-110. doi: 10.1016/j.cell.2007.02.036.
6
Structure of dimeric SecA, the Escherichia coli preprotein translocase motor.大肠杆菌前体蛋白转位酶马达二聚体SecA的结构
J Mol Biol. 2007 Mar 9;366(5):1545-57. doi: 10.1016/j.jmb.2006.12.049. Epub 2006 Dec 23.
7
Crystal structure of the translocation ATPase SecA from Thermus thermophilus reveals a parallel, head-to-head dimer.嗜热栖热菌转运ATP酶SecA的晶体结构揭示了一种平行的、头对头的二聚体。
J Mol Biol. 2006 Dec 1;364(3):248-58. doi: 10.1016/j.jmb.2006.09.061. Epub 2006 Sep 29.
8
A novel dimer interface and conformational changes revealed by an X-ray structure of B. subtilis SecA.枯草芽孢杆菌SecA的X射线结构揭示的新型二聚体界面和构象变化。
J Mol Biol. 2006 Dec 1;364(3):259-65. doi: 10.1016/j.jmb.2006.08.044. Epub 2006 Aug 22.
9
Characterization of three areas of interactions stabilizing complexes between SecA and SecB, two proteins involved in protein export.SecA和SecB是参与蛋白质输出的两种蛋白质，对二者之间稳定复合物的三个相互作用区域的表征。
Protein Sci. 2006 Jun;15(6):1379-86. doi: 10.1110/ps.062141006.
10
SecA supports a constant rate of preprotein translocation.SecA支持前体蛋白以恒定速率进行转运。
J Biol Chem. 2006 Jun 9;281(23):15709-13. doi: 10.1074/jbc.M600205200. Epub 2006 Apr 6.
Zotero 插件