Das Suchismita, Garver Lindsey, Ramirez Jose Ruiz, Xi Zhiyong, Dimopoulos George
Malaria Research Institute, Bloomberg School of Public Health, John Hopkins University, USA.
J Vis Exp. 2007(5):220. doi: 10.3791/220. Epub 2007 Jul 4.
The purpose of this procedure is to infect the Aedes mosquito with dengue virus in a laboratory condition and examine the infection level and dynamic of the virus in the mosquito tissues. This protocol is routinely used for studying mosquito-virus interactions, especially for identification of novel host factors that are able to determine vector competence. The entire experiment must be conducted in a BSL2 laboratory. Similar to Plasmodium falciparum infections, proper attire including gloves and lab coat must be worn at all times. After the experiment, all the materials that came in contact with the virus need to be treated with 75% ethanol and bleached before proceeding with normal washing. All other materials need to be autoclaved before discarding them.
本程序的目的是在实验室条件下用登革病毒感染伊蚊，并检测病毒在蚊虫组织中的感染水平及动态变化。该方案常用于研究蚊虫与病毒的相互作用，特别是用于鉴定能够决定媒介能力的新型宿主因子。整个实验必须在生物安全二级（BSL2）实验室进行。与恶性疟原虫感染类似，必须始终穿戴包括手套和实验室工作服在内的适当防护装备。实验结束后，所有与病毒接触过的材料在进行正常清洗之前，都需要用75%乙醇处理并进行漂白。所有其他材料在丢弃之前都需要进行高压灭菌。
