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褪黑素可减轻辐射诱导的氧化损伤对大鼠晶状体造成的氧化应激。

Melatonin reduces oxidative stress in the rat lens due to radiation-induced oxidative injury.

作者信息

Taysi Seyithan, Memisogullari Ramazan, Koc Mehmet, Yazici Ahmet Taylan, Aslankurt Murat, Gumustekin Kenan, Al Behcet, Ozabacigil Fatma, Yilmaz Adnan, Tahsin Ozder Hasan

机构信息

Department of Biochemistry and Clinical Biochemistry, Gaziantep University, School of Medicine, Gaziantep, Turkey.

出版信息

Int J Radiat Biol. 2008 Oct;84(10):803-8. doi: 10.1080/09553000802390932.

Abstract

PURPOSE

The aim of this study was to evaluate the antioxidant role of melatonin against radiation-induced cataract in the rat lens after total cranial irradiation with a single 5 Gray (Gy) dose of gamma irradiation.

MATERIALS AND METHODS

Twenty-eight Sprague-Dawley rats were used for the experiment.The rats were randomly divided into four equal groups. The control group did not receive melatonin or irradiation but received both 0.1 ml physiological saline intraperitoneally and sham irradiation. The irradiation (IR) group received 5 Gy gamma irradiation to the total cranium as a single dose plus 0.1 ml physiological saline intraperitoneally. The melatonin plus IR group received irradiation to the total cranium plus 5 mg/kg/day melatonin intraperitoneally. The melatonin group received only 5 mg/kg/day melatonin plus sham-irradiation. Biochemical parameters measured in murine lenses were carried out using spectrophotometric techniques.

RESULTS

Lens antioxidant capacity, as measured by levels of total superoxide scavenger activity (TSSA), non-enzymatic superoxide scavenger activity (NSSA) and glutathione reductase (GRD) activity, significantly increased in melatonin, control and melatonin plus IR groups when compared with the IR group. Lens glutathione-S-transferase (GST) activity significantly increased in control and melatonin groups when compared with the IR group. Lens malondialdehyde (MDA) levels significantly increased in the IR group when compared with control, melatonin and melatonin plus IR groups. Lens TSSA and NSSA activities significantly decreased in control and melatonin plus IR groups when compared with the melatonin group. Lens GST activity significantly increased in the control group when compared with melatonin plus IR group. Lens GRD activity significantly increased in melatonin and melatonin plus IR groups when compared with control group.

CONCLUSIONS

Melatonin reduces oxidative stress markers and augments anti-oxidant capacity in the rat lens.

摘要

目的

本研究旨在评估褪黑素对单次5格雷(Gy)剂量伽马射线全颅照射后大鼠晶状体辐射诱导性白内障的抗氧化作用。

材料与方法

28只Sprague-Dawley大鼠用于实验。大鼠被随机分为四组,每组数量相等。对照组未接受褪黑素或照射,而是腹腔注射0.1毫升生理盐水并接受假照射。照射(IR)组接受单次剂量5 Gy的全颅伽马射线照射,并腹腔注射0.1毫升生理盐水。褪黑素加IR组接受全颅照射并腹腔注射5毫克/千克/天的褪黑素。褪黑素组仅接受5毫克/千克/天的褪黑素加假照射。使用分光光度技术测量小鼠晶状体中的生化参数。

结果

与IR组相比,褪黑素组、对照组和褪黑素加IR组中,通过总超氧化物清除剂活性(TSSA)、非酶促超氧化物清除剂活性(NSSA)和谷胱甘肽还原酶(GRD)活性水平测量的晶状体抗氧化能力显著增加。与IR组相比,对照组和褪黑素组中晶状体谷胱甘肽-S-转移酶(GST)活性显著增加。与对照组、褪黑素组和褪黑素加IR组相比,IR组中晶状体丙二醛(MDA)水平显著增加。与褪黑素组相比,对照组和褪黑素加IR组中晶状体TSSA和NSSA活性显著降低。与褪黑素加IR组相比,对照组中晶状体GST活性显著增加。与对照组相比,褪黑素组和褪黑素加IR组中晶状体GRD活性显著增加。

结论

褪黑素可降低大鼠晶状体中的氧化应激标志物并增强抗氧化能力。

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