Yuan Weiwei, Yang Jiyuan, Kopecková Pavla, Kopecek Jindrich
Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City, Utah 84112, USA.
J Am Chem Soc. 2008 Nov 26;130(47):15760-1. doi: 10.1021/ja805634x.
Enzyme-based hybrid hydrogels were prepared by covalently incorporating an adenylate kinase mutant, possessing two thiol groups, into HPMA copolymer- or PEG-based hydrogel structures. The nanoscale conformational change of enzyme, triggered by substrate recognition, translated into macroscopic motion of hydrogels.
通过将具有两个硫醇基团的腺苷酸激酶突变体共价掺入基于HPMA共聚物或聚乙二醇的水凝胶结构中，制备了基于酶的混合水凝胶。由底物识别引发的酶的纳米级构象变化转化为水凝胶的宏观运动。
