Regidor-Cerrillo J, Gómez-Bautista M, Pereira-Bueno J, Aduriz G, Navarro-Lozano V, Risco-Castillo V, Férnandez-García A, Pedraza-Díaz S, Ortega-Mora L M
SALUVET, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain.
Parasitology. 2008 Dec;135(14):1651-9. doi: 10.1017/S003118200800509X. Epub 2008 Nov 4.
Neospora caninum is a cyst-forming parasite that causes abortion in cattle. Despite this parasite's ubiquitous distribution and wide host range, the number of N. caninum isolates obtained to date is limited. In vitro isolation of the parasite is arduous and often unsuccessful. In addition, most isolates have been obtained from clinically affected hosts and therefore could be biased towards more virulent isolates. In this report, an improved isolation approach from transplacentally infected newborn calves was undertaken and 9 new isolates were obtained. Moreover, a microsatellite technique was applied to investigate the genetic diversity of these isolates. Most isolates showed specific genetic profiles. However, the Nc-Spain10 isolate was identical to the previously described Nc-Spain1H isolate and Nc-Spain3H was identical to Nc-Spain4H. These isolates were likely to have identical genotypes because they were isolated from distinct calves of the same herd. Future pathogenic characterization of these isolates will contribute to the investigation of the relationship between isolate virulence and the outcome of infection, as well as other epidemiological features, such as transmission.
犬新孢子虫是一种形成包囊的寄生虫，可导致牛流产。尽管这种寄生虫分布广泛且宿主范围广，但迄今为止获得的犬新孢子虫分离株数量有限。该寄生虫的体外分离工作艰巨且常常不成功。此外，大多数分离株是从临床感染宿主中获得的，因此可能偏向于毒性更强的分离株。在本报告中，采用了一种改进的从经胎盘感染的新生犊牛中分离的方法，并获得了9个新的分离株。此外，应用微卫星技术研究了这些分离株的遗传多样性。大多数分离株显示出特定的遗传特征。然而，Nc-Spain10分离株与先前描述的Nc-Spain1H分离株相同，Nc-Spain3H与Nc-Spain4H相同。这些分离株可能具有相同的基因型，因为它们是从同一牛群的不同犊牛中分离出来的。对这些分离株未来的致病性特征进行研究，将有助于调查分离株毒力与感染结果之间的关系，以及其他流行病学特征，如传播情况。
