Genetics Unit, Department of Biology, Universidad Autónoma de Madrid (UAM), Madrid, Spain.
Fertil Steril. 2009 Dec;92(6):1915-9. doi: 10.1016/j.fertnstert.2008.08.136. Epub 2008 Nov 5.
To analyze any possible dynamic correlation between sperm DNA fragmentation and sperm viability.
The rate of viability loss and the rate of increase of the frequency of sperm cells with fragmented DNA were determined at 0, 1.5, 4.5, and 24.0 hours after thawing samples from donors with proven fertility.
Academic biology and reproductive medicine centers.
PATIENT(S): Fifteen male donors with proven fertility for a maximum of six births at the reproductive medicine center.
INTERVENTION(S): None.
MAIN OUTCOME MEASURE(S): Sperm DNA fragmentation and viability dynamics expressed as logarithmic coefficients of change.
RESULT(S): The dynamics of sperm DNA fragmentation and sperm viability adjusted to a logarithmic function with an initial highest velocity that progressively decreases. Nevertheless, the rates were not statistically significantly correlated.
CONCLUSION(S): In the short term, dynamic dysfunction of membrane permeability does not result in DNA fragmentation and thus must be considered as independent parameters of sperm quality.
分析精子 DNA 碎片化与精子活力之间可能存在的动态相关性。
在解冻供体样本后 0、1.5、4.5 和 24.0 小时，确定具有已知生育能力的供体样本的活力丧失率和具有碎片化 DNA 的精子细胞频率增加率。
学术生物学和生殖医学中心。
生殖医学中心最多有 6 次生育经历的 15 名具有已知生育能力的男性供体。
无。
以对数变化系数表示的精子 DNA 碎片化和活力动态。
精子 DNA 碎片化和精子活力的动力学调整为对数函数，初始速度最高，逐渐降低。然而，这些速度没有统计学显著相关性。
在短期内，膜通透性的动态功能障碍不会导致 DNA 碎片化，因此必须被视为精子质量的独立参数。
