Division of Reproductive Medicine, Clinical Embryology Laboratory, Kasturba Medical College Manipal University, Manipal 576 104, India.
J Assist Reprod Genet. 2012 Jun;29(6):557-63. doi: 10.1007/s10815-012-9742-x. Epub 2012 Mar 13.
DNA damage may occur during sperm processing, thereby negatively influencing fertilizing ability of the sperm. The present study was designed to compare the effectiveness of gradient and swim-up, either alone or in combination, to eliminate sperm with DNA damage.
A total of 51 subjects visiting the University infertility clinic with normozoospermic parameters, oligozoospermia and teratozoospermia were included. Semen characteristics were analysed by standard criteria; Terminal deoxy nucelotidyl transferase mediated dUTP nick end labeling assay was employed for DNA damage assessment.
The percentage of TUNEL positive sperm after sperm processing was significantly lower in normozoospermic (P < 0.05), oligozoospermic (P < 0.001) and teratozoospermic samples (P < 0.01). No difference was observed in the incidence of TUNEL positive sperm between the various techniques, suggesting that they are comparable.
Sperm preparation has been found to result in enrichment of sperm with intact chromatin, which is likely to improve the chances of achieving a viable pregnancy.
精子处理过程中可能会发生 DNA 损伤，从而降低精子的受精能力。本研究旨在比较梯度离心法和上游法单独或联合应用对消除具有 DNA 损伤的精子的效果。
共纳入 51 例在大学不孕不育诊所就诊的具有正常精子参数、少精子症和畸形精子症的患者。采用标准方法分析精液特征；采用末端脱氧核苷酸转移酶介导的 dUTP 缺口末端标记法评估 DNA 损伤。
精子处理后 TUNEL 阳性精子的百分比在正常精子症组（P < 0.05）、少精子症组（P < 0.001）和畸形精子症组（P < 0.01）中显著降低。不同技术之间 TUNEL 阳性精子的发生率无差异，表明它们具有可比性。
精子处理后发现精子中富含完整染色质，这可能增加实现可存活妊娠的机会。
