一种用于检测两种新鉴定的人类KI和WU多瘤病毒的单管实时PCR检测方法。
A single-tube, real-time PCR assay for detection of the two newly characterized human KI and WU polyomaviruses.
作者信息
Lindau Cecilia, Tiveljung-Lindell Annika, Goh Shan, Ramqvist Torbjörn, Allander Tobias
机构信息
Department of Clinical Microbiology, Karolinska University Hospital, Stockholm, Sweden.
出版信息
J Clin Virol. 2009 Jan;44(1):24-6. doi: 10.1016/j.jcv.2008.09.006. Epub 2008 Nov 5.
BACKGROUND
Three new human polyomaviruses have been recently described, and investigating their in vivo biology and pathogenicity will require sensitive and rational detection assays.
OBJECTIVES
To develop and evaluate a sensitive and rational assay for detection of the newly identified KI and WU polyomaviruses.
STUDY DESIGN
A single-tube, dual-probe, real-time PCR assay for simultaneous detection and discrimination of KI and WU polyomaviruses was developed.
RESULTS
The assay had near single-molecule sensitivity for both viruses and no cross-reactivity was observed. A panel of 637 nasopharyngeal aspirates was screened, resulting in a frequency of 1.4% for KIPyV and 1.3% for WUPyV.
CONCLUSIONS
The dual-probe assay provides a rational approach for further studies of KIPyV and WUPyV.
背景
最近发现了三种新的人类多瘤病毒，对其体内生物学特性和致病性进行研究需要灵敏且合理的检测方法。
目的
开发并评估一种灵敏且合理的检测新发现的KI和WU多瘤病毒的方法。
研究设计
开发了一种单管双探针实时PCR检测方法，用于同时检测和鉴别KI和WU多瘤病毒。
结果
该检测方法对两种病毒均具有近乎单分子的灵敏度，未观察到交叉反应。对637份鼻咽抽吸物样本进行筛查，结果显示KI多瘤病毒（KIPyV）的检出率为1.4%，WU多瘤病毒（WUPyV）的检出率为1.3%。
结论
双探针检测方法为进一步研究KIPyV和WUPyV提供了一种合理的途径。
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