Department of Public Health and Microbiology, Virology Unit, University of Turin, Via Santena 9, 10126 Turin, Italy.
J Virol Methods. 2009 Dec;162(1-2):69-74. doi: 10.1016/j.jviromet.2009.07.016. Epub 2009 Jul 29.
Recently, polyomaviruses KI and WU were identified in the airways of patients with acute respiratory symptoms. The epidemiology and pathogenesis of these two viruses are not fully understood, and the development of molecular assays, such as Real Time PCR, was useful for examining their biology and role in different clinical syndromes. The evaluation of different target regions for the amplification of polyomaviruses KI and WU, comparing published primer/probe sets and sets designed in the laboratory is described and was used for testing 175 clinical specimens (84 stools and 91 tonsils). The results showed that the laboratory designs were more sensitive for the detection of polyomaviruses KI and WU DNA in clinical samples. The choice of the primer/probe set, and primarily of the region for amplification, may be relevant for understanding the pathogenic role of viruses such as polyomaviruses KI and WU.
最近，在急性呼吸道症状患者的气道中发现了多瘤病毒 KI 和 WU。这两种病毒的流行病学和发病机制尚未完全了解，分子检测方法（如实时 PCR）的发展有助于研究它们的生物学特性及其在不同临床综合征中的作用。本文描述了对不同靶区域进行扩增的评估，比较了已发表的引物/探针集和实验室设计的集，并用于检测 175 份临床标本（84 份粪便和 91 份扁桃体）。结果表明，实验室设计对临床样本中多瘤病毒 KI 和 WU DNA 的检测更敏感。引物/探针集的选择，主要是扩增区域的选择，可能与理解多瘤病毒 KI 和 WU 等病毒的致病作用有关。
