Li Hong-Bin, Zhang Lei-Ping, Chen San-Feng
The State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100094, PR China.
Int J Syst Evol Microbiol. 2008 Nov;58(Pt 11):2582-8. doi: 10.1099/ijs.0.65711-0.
Five Gram-negative, rod-shaped, moderately halophilic and denitrifying strains, designated XK1(T), XK2, XK3, XK4 and XK5, were isolated from a saline and alkaline soil in Korla, north-western China. These isolates could grow anaerobically using either nitrate or nitrite as terminal electron acceptors and produced gas from nitrate vigorously. A comparison and phylogenetic analysis of 16S rRNA gene sequences placed these isolates in the genus Halomonas within the family Halomonadaceae. The isolates shared the highest 16S rRNA gene sequence similarities with Halomonas ventosae Al12(T )(95.6 %), Halomonas alimentaria YKJ-16(T) (95.5 %) and Halomonas shengliensis SL014B-85(T) (95.2 %) (values determined by mega 3.1; direct comparison results with GenBank were even lower, not >/=94 %). Sequence similarities with other recognized species were below 95.0 %, far below the 97.0 % threshold generally accepted for the delineation of separate species. BOX-PCR fingerprinting and DNA-DNA hybridization showed high similarities among the five strains which indicated they were members of the same species. The major fatty acids were C(18 : 1)omega8t, C(16 : 0) and C(18 : 1)omega7t. The DNA G+C content was 65.3 mol%. All the results of the phenotypic and genetic analyses supported the hypothesis that the five new strains represent a novel species within the genus Halomonas, for which the name Halomonas korlensis sp. nov. is proposed. The type strain is XK1(T) (=CGMCC 1.6981(T)=DSM 19633(T)).
从中国西北部库尔勒的盐碱土中分离出五株革兰氏阴性、杆状、中度嗜盐且能进行反硝化作用的菌株，分别命名为XK1(T)、XK2、XK3、XK4和XK5。这些分离株能够以硝酸盐或亚硝酸盐作为末端电子受体进行厌氧生长，并能从硝酸盐中剧烈产生气体。对16S rRNA基因序列进行比较和系统发育分析，将这些分离株归入盐单胞菌科的盐单胞菌属。这些分离株与通风盐单胞菌Al12(T)（95.6%）、食源盐单胞菌YKJ-16(T)（95.5%）和胜利盐单胞菌SL014B-85(T)（95.2%）共享最高的16S rRNA基因序列相似性（由mega 3.1确定的值；与GenBank的直接比较结果更低，不大于/等于94%）。与其他已确认物种的序列相似性低于95.0%，远低于通常用于划分不同物种的97.0%阈值。BOX-PCR指纹图谱和DNA-DNA杂交显示这五株菌株之间具有高度相似性，表明它们是同一物种的成员。主要脂肪酸为C(18 : 1)ω8t、C(16 : 0)和C(18 : 1)ω7t。DNA G+C含量为65.3 mol%。表型和遗传分析的所有结果支持了这五株新菌株代表盐单胞菌属内一个新物种的假设，为此提出盐单胞菌库尔勒新种（Halomonas korlensis sp. nov.）。模式菌株为XK1(T)（=CGMCC 1.6981(T)=DSM 19633(T)）。
