Arenas María, Bañón Patricia I, Copa-Patiño José L, Sánchez-Porro Cristina, Ventosa Antonio, Soliveri Juan
Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Alcalá, 28871 Alcalá de Henares, Madrid, Spain.
Int J Syst Evol Microbiol. 2009 Mar;59(Pt 3):578-82. doi: 10.1099/ijs.0.003509-0.
A Gram-negative, heterotrophic, aerobic, pale orange-pigmented, non-endospore-forming and motile bacterial strain, designated strain SP8(T), was isolated from a salty water sample from the solar salterns of Santa Pola, located on the Mediterranean coast of Spain. The strain grew optimally at 37 degrees C, pH 6.5 and in the presence of 10 % NaCl. A polyphasic taxonomic study was conducted in order to characterize the strain in detail. Phylogenetic analyses based on 16S rRNA gene sequence comparisons indicated that strain SP8(T) clustered within the branch constituted by species of the genus Halomonas. The closest phylogenetic neighbours of strain SP8(T) were Halomonas muralis LMG 20969(T) (96.0 % sequence similarity), Halomonas pantelleriensis AAP(T) (95.9 %) and Halomonas campaniensis 5AG(T) (95.8 %). Phenotypic features, the fatty acid profile and the DNA G+C content of the novel strain further supported its placement in the genus Halomonas. On the basis of phenotypic, chemotaxonomic and phylogenetic distinctiveness, it is suggested that strain SP8(T) represents a novel species for which the name Halomonas ilicicola sp. nov. is proposed. The type strain is SP8(T) (=CECT 7331(T)=CCM 7522(T)=DSM 19980(T)).
从西班牙地中海沿岸圣波拉盐场的一份咸水样品中分离出一株革兰氏阴性、异养、需氧、浅橙色色素、不形成芽孢且具运动性的细菌菌株，命名为菌株SP8(T)。该菌株在37℃、pH 6.5以及10% NaCl存在的条件下生长最佳。为详细表征该菌株，开展了多相分类研究。基于16S rRNA基因序列比较的系统发育分析表明，菌株SP8(T)聚集在由嗜盐单胞菌属物种构成的分支内。菌株SP8(T)最接近的系统发育邻居是墙壁嗜盐单胞菌LMG 20969(T)（序列相似性为96.0%）、潘泰莱里亚嗜盐单胞菌AAP(T)（95.9%）和坎帕尼亚嗜盐单胞菌5AG(T)（95.8%）。该新菌株的表型特征、脂肪酸谱和DNA G+C含量进一步支持其归入嗜盐单胞菌属。基于表型、化学分类和系统发育的独特性，建议菌株SP8(T)代表一个新物种，为此提出名称为滨海嗜盐单胞菌（Halomonas ilicicola sp. nov.）。模式菌株为SP8(T)（=CECT 7331(T)=CCM 7522(T)=DSM 19980(T)）。
