Wang Yu, Wu Yue-Hong, Wang Chun-Sheng, Xu Xue-Wei, Oren Aharon, Zhu Xu-Fen, Wu Min
College of Life Sciences, Zhejiang University, Hangzhou, PR China.
Int J Syst Evol Microbiol. 2008 Dec;58(Pt 12):2855-8. doi: 10.1099/ijs.0.2008/000729-0.
A Gram-negative, aerobic, motile, halophilic bacterium, designated strain BC7(T), was isolated from a salt mine in north-western China and was subjected to a polyphasic taxonomic characterization. The isolate was able to grow in the presence of 0.5-20 % (w/v) NaCl and at pH 6.0-9.0 and 4-48 degrees C; optimum growth was observed with 3 % (w/v) NaCl and at pH 7.0 and 30 degrees C. Cells were long rods, 0.8-1.2 microm wide and 4.0-6.0 microm long. The major fatty acids were C(18 : 1)omega7c, C(16 : 0) and C(16 : 0)omega7c. The DNA G+C content was 65.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BC7(T) belonged to the genus Halomonas and showed highest sequence similarity to the type strain of Halomonas pacifica (99.2 %). Levels of DNA-DNA relatedness between strain BC7(T) and H. pacifica CGMCC 1.2314(T) and Halomonas taeanensis DSM 16463(T) were 51 and 38 %, respectively. On the basis of phenotypic and genotypic data, strain BC7(T) is considered to represent a novel species of the genus Halomonas, for which the name Halomonas salifodinae sp. nov. is proposed. The type strain is BC7(T) (=CGMCC 1.6774(T) =JCM 14803(T)).
从中国西北部的一个盐矿中分离出一株革兰氏阴性、需氧、运动、嗜盐细菌，命名为BC7(T)菌株，并对其进行了多相分类学鉴定。该菌株能够在0.5-20%（w/v）NaCl、pH值6.0-9.0和4-48℃的条件下生长；在3%（w/v）NaCl、pH值7.0和30℃时观察到最佳生长。细胞为长杆状，宽0.8-1.2微米，长4.0-6.0微米。主要脂肪酸为C(18 : 1)ω7c、C(16 : 0)和C(16 : 0)ω7c。DNA G+C含量为65.5 mol%。基于16S rRNA基因序列的系统发育分析表明，BC7(T)菌株属于嗜盐单胞菌属，与太平洋嗜盐单胞菌模式菌株的序列相似性最高（99.2%）。BC7(T)菌株与太平洋嗜盐单胞菌CGMCC 1.2314(T)和泰安嗜盐单胞菌DSM 16463(T)之间的DNA-DNA相关性水平分别为51%和38%。基于表型和基因型数据，BC7(T)菌株被认为代表嗜盐单胞菌属的一个新物种，为此提出了新名称盐矿嗜盐单胞菌（Halomonas salifodinae sp. nov.）。模式菌株为BC7(T)（=CGMCC 1.6774(T)=JCM 14803(T)）。
