Wu Gang, Wu Xiao-Qing, Wang Ya-Nan, Chi Chang-Qiao, Tang Yue-Qin, Kida Kenji, Wu Xiao-Lei, Luan Zhao-Kun
State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, PR China.
Int J Syst Evol Microbiol. 2008 Dec;58(Pt 12):2859-65. doi: 10.1099/ijs.0.65746-0.
A Gram-negative, moderately halophilic, short rod-shaped, aerobic bacterium with peritrichous flagellae, strain DQD2-30(T), was isolated from a soil sample contaminated with crude oil from the Daqing oilfield in Heilongjiang Province, north-eastern China. The novel strain was capable of growth at NaCl concentrations of 1-15 % (w/v) [optimum at 5-10 % (w/v)]. Phylogenetic analyses based on 16S rRNA gene sequences showed that the novel strain belonged to the genus Halomonas in the class Gammaproteobacteria; the highest 16S rRNA gene sequence similarities were with Halomonas desiderata DSM 9502(T) (98.8 %), Halomonas campisalis A4(T) (96.6 %) and Halomonas gudaonensis CGMCC 1.6133(T) (95.1 %). The major cellular fatty acids of strain DQD2-30(T) were C(18 : 1)omega7c (43.97 %), C(19 : 0 )cyclo omega8c (23.37 %) and C(16 : 0) (14.83 %). The predominant respiratory lipoquinone was ubiquinone with nine isoprene units (Q9). The DNA G+C content was 67.0 mol%. The DNA-DNA hybridization values of strain DQD2-30(T) with the most closely related species of the genus Halomonas were 51.8 %, 28.4 % and 23.5 % for H. desiderata, H. campisalis and H. gudaonensis, respectively. Based on these analyses, strain DQD2-30(T )(=CGMCC 1.6443(T)=LMG 23896(T)) is proposed to represent the type strain of a novel species, Halomonas daqingensis sp. nov.
从中国东北黑龙江省大庆油田原油污染土壤样本中分离出一株革兰氏阴性、中度嗜盐、短杆状、具周生鞭毛的需氧细菌，菌株DQD2-30(T)。该新菌株能够在1-15%（w/v）的NaCl浓度下生长[最适浓度为5-10%（w/v）]。基于16S rRNA基因序列的系统发育分析表明，该新菌株属于γ-变形菌纲的嗜盐单胞菌属；16S rRNA基因序列最高相似性分别与渴望嗜盐单胞菌DSM 9502(T)（98.8%）、盐沼嗜盐单胞菌A4(T)（96.6%）和古岛嗜盐单胞菌CGMCC 1.6133(T)（95.1%）。菌株DQD2-30(T)的主要细胞脂肪酸为C(18 : 1)ω7c（43.97%）、C(19 : 0)环ω8c（23.37%）和C(16 : 0)（14.83%）。主要呼吸醌为含9个异戊二烯单位的泛醌（Q9）。DNA G+C含量为67.0 mol%。菌株DQD2-30(T)与嗜盐单胞菌属最密切相关物种的DNA-DNA杂交值，对于渴望嗜盐单胞菌、盐沼嗜盐单胞菌和古岛嗜盐单胞菌分别为51.8%、28.4%和23.5%。基于这些分析，提议菌株DQD2-30(T)(=CGMCC 1.6443(T)=LMG 23896(T))代表一个新物种大庆嗜盐单胞菌（Halomonas daqingensis sp. nov.）的模式菌株。
